Role of cap 1 and cap 2 2ʼ-O-ribose methylation in Drosophila development and nervous system function and LIM Domain Only Proteins, LMO1 and LMO4 in Hematopoiesis

Edginton-White, Benjamin (2014). Role of cap 1 and cap 2 2ʼ-O-ribose methylation in Drosophila development and nervous system function and LIM Domain Only Proteins, LMO1 and LMO4 in Hematopoiesis. University of Birmingham. M.Res.

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Abstract

LIM Domain Only Proteins, LMO1 and LMO4 in Hematopoiesis
Role of cap 1 and cap 2 2ʼ-O-ribose methylation in Drosophila development and nervous system function (Page 1) - The presence of cap 1 and cap 2 2ʼ-O- ribose methylation on the second and third nucleotide of mRNA has been known for more than 30 years however studies into the role of these methylations are very limited.
Research has shown that Cap 1 methylation may be related to translational efficiency however no studies have shown a role for Cap 2 methylation. This study aims to extend the understanding of the function of these methylations in the neural development of Drosophila.

LIM Domain Only Proteins, LMO1 and LMO4 in Hematopoiesis (Page 72) - The
LIM Domain Only (LMO) proteins have been shown to play a major role in cellular differentiation and development at the embryonic stage and in many human cancers.
The aim of this study was to develop tools to perform overexpression and knockdown experiments with LMO1 and LMO4 proteins in murine early stage hematopoietic progenitors and ES cells in an attempt to identify their function. Preliminary results show a possible apoptotic effect of LMO4 knockdown in myeloid progenitor cells.
This study provides the basis for future research into these proteins.

Type of Work:

Thesis (Masters by Research > M.Res.)

Award Type:

Masters by Research > M.Res.

Supervisor(s):