Investigations in MALDI-MSI using a high repetition rate laser

Steven, Rory Thomas (2014). Investigations in MALDI-MSI using a high repetition rate laser. University of Birmingham. Ph.D.

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Abstract

Within these studies the properties of a high repetition rate (≤ 25,000 Hz) Nd:YVO4 laser were investigated with a view to improving the throughput and quality of information obtained from MALDI MS data collected in continuous raster sampling mode.
Initially, the nature of the laser beam profile was investigated and a novel fluorometric method developed for imaging these profiles. Analysis of thin film samples of α-cyano-4-hydroxycinnamic acid (CHCA) and lipid standard phophatidylcholine (PC) 34:1 were carried out. Under most conditions a lower repetition rate and slow stage raster speed were found to be optimal. However, in tissue based investigations repetition rates of 5-10 kHz and faster raster speeds were found to increase the detected ion intensity. Subsequent to this, the use of para-nitroaniline (PNA) as an effective matrix for high-repetition rate laser MALDI MSI applications was investigated and compared to CHCA. PNA was found to provide high quality MSI data, comparable to or better than that obtained with CHCA. The utility of CHCA and PNA were then investigated for the repeat analysis of single tissue sections with a view to increasing the amount of information obtained. Up to five analyses of the same tissue section were demonstrated. Repeat analysis was then applied to the acquisition of both lipid and protein data from a single tissue section using multiple matrices and tissue washing.

Type of Work:	Thesis (Doctorates > Ph.D.)
Award Type:	Doctorates > Ph.D.
Supervisor(s):	Supervisor(s) Email ORCID Bunch, Josephine UNSPECIFIED UNSPECIFIED Rainger, G.E. UNSPECIFIED UNSPECIFIED Kaban, Ata UNSPECIFIED UNSPECIFIED Nash, G. B. (Gerard B.) UNSPECIFIED UNSPECIFIED
Licence:
College/Faculty:	Colleges (2008 onwards) > College of Engineering & Physical Sciences
School or Department:	School of Chemistry
Funders:	Engineering and Physical Sciences Research Council
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology
URI:	http://etheses.bham.ac.uk/id/eprint/5392

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