Diversity among monocyte derived stromal cells

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http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/1645 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/1645 http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/1645 archive 4031 disk0/00/00/06/79 2010-05-19 11:07:24 2019-01-23 09:52:38 2011-07-19 16:21:23 thesis show 0 Fairclough Marianne Elizabeth marianne@davefairclough.co.uk Salmon M Curnow SJ Wallace Graham University of Birmingham Diversity among monocyte derived stromal cells unpub RC QR180 10col_mede Work related to this thesis has been published as: Curnow SJ, Fairclough M, Schmutz C, Kissane S, Denniston AKO, et al. (2010) Distinct types of fibrocyte can differentiate from mononuclear cells in the presence and absence of serum. PLoS ONE 5(3): e9730. doi:10.1371/journal.pone.0009730 http://eprints.bham.ac.uk/337/ Fibrocytes are monocyte-derived cells that morphologically look like fibroblasts, express both stromal and haematopoietic markers, and have been reported as being involved in wound healing and fibrosis. In-vitro derived fibrocytes can be differentiated in both serum-containing and serum-free environments and we wanted to study the relationship between these two fibrocytes; which potentially could be involved at different time points at a wound healing site. To investigate the relationship between serum-free and serum-containing derived fibrocytes monocytes were differentiated without serum. When these cells were placed in a serum-containing environment they became round, losing their fibroblast-like morphology. However when the reverse experiment was done on fibrocytes derived in a serum-containing environment there was no apparent effect on their morphology. The relationships between these two fibrocytes, as well as macrophages and fibroblasts was also examined using transcriptome analysis of 37000 genes, clustering the samples based on all the genes, and identifying those that were significantly different between the populations. This demonstrated that both fibrocyte populations are distinct from each other, as well as from both fibroblasts and macrophages. These data demonstrate that these two fibrocytes have different characteristics, suggesting that they may have different roles in the modulation of fibrosis in inflammation. 2010-07 2009-09 graduation eng author unchecked This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. public 306 University of Birmingham School of Immunity and Infection d_ph va mrc 2010-07 graduation