Dynamic histone modifications at the promoters of Hox genes in embryonic stem cells

483 29 1159 9 1675 document 1159 Stower09PhD.pdf application/pdf a243071166ff5d6512f987283ee5fa59 MD5 6858489 2011-07-19 16:18:12 http://etheses.bham.ac.uk//id/eprint/483/1/Stower09PhD.pdf 483 1 1 application/pdf application/pdf en public

Stower09PhD.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/24666 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/24666 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/24666 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/24667 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/24667 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/24667 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/24938 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/24938 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/24938 1160 6 1676 document 1160 Decl_IS_Stower09PhD.jpg image/jpeg 7abd0c860a4704975b88c8f6a06b614f MD5 62611 2011-07-19 16:18:12 http://etheses.bham.ac.uk//id/eprint/483/2/Decl_IS_Stower09PhD.jpg 483 2 2 image/jpeg image/jpeg en staffonly

Decl_IS_Stower09PhD.jpg

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/24668 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/24668 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/24668 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/24669 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/24669 http://eprints.org/relation/hasmediumThumbnailVersion http://etheses.bham.ac.uk/id/document/24669 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/24670 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/24670 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/24670 24670 2 51601 document 24670 preview.jpg image/jpeg 1a37b0aac738c99b27c9735b19922bf9 MD5 5175 2013-01-29 14:57:44 http://etheses.bham.ac.uk//id/eprint/483/17/preview.jpg 483 17 17 image/jpeg image/jpg Image thumbnails conversion from image/jpeg to thumbnail_preview en staffonly

preview.jpg

medium.jpg

preview.jpg

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/1159 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/1159 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/1159 9921 2 12556 document 9921 indexcodes.txt text/plain 27014dcd3b5765398e942294e3c4bdf6 MD5 43721 2011-07-20 00:44:34 http://etheses.bham.ac.uk//id/eprint/483/8/indexcodes.txt 483 8 8 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en public

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/1159 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/1159 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/1159 24938 2 52020 document 24938 indexcodes.txt text/plain 74b1d17a863da5e3ea0a4556f3ae2440 MD5 43721 2013-01-29 17:05:57 http://etheses.bham.ac.uk//id/eprint/483/18/indexcodes.txt 483 18 18 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en public

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/1159 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/1159 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/1159 24666 2 51596 document 24666 small.png image/png 4b7449a572776eec501c5e37303a255a MD5 2998 2013-01-29 14:57:42 http://etheses.bham.ac.uk//id/eprint/483/13/small.png 483 13 13 image/png thumbnail_small Thumbnail Documents conversion from application/pdf to thumbnail_small en public

small.png

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/1159 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/1159 http://eprints.org/relation/issmallThumbnailVersionOf http://etheses.bham.ac.uk/id/document/1159 24668 2 51599 document 24668 small.jpg image/jpeg c407afee7d041da3493c42dde8d851be MD5 275 2013-01-29 14:57:44 http://etheses.bham.ac.uk//id/eprint/483/15/small.jpg 483 15 15 image/jpeg image/jpg Image thumbnails conversion from image/jpeg to thumbnail_small en staffonly

small.jpg

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/1160 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/1160 http://eprints.org/relation/issmallThumbnailVersionOf http://etheses.bham.ac.uk/id/document/1160 archive 4031 disk0/00/00/04/83 2010-01-14 12:13:35 2019-01-23 09:52:14 2011-07-19 16:18:12 thesis show 0 Stower Hannah Mary Turner Bryan M. University of Birmingham Dynamic histone modifications at the promoters of Hox genes in embryonic stem cells unpub R1 QH426 10col_mede Histone modifications have been closely associated with changing levels of gene expression, but their role in determining, or possibly predicting, patterns of expression is uncertain. Here, the link between histone modifications and Hoxb gene expression in mouse embryonic stem (ES) cells was explored. Levels of the “active” modifications H3K9ac and H3K4me3 at Hoxb promoters varied widely from gene to gene, but were closely correlated in ES cells. Contrastingly, the repressive modification H3K27me3 was found at equivalent levels across the cluster. Treatment with the histone deacetylase inhibitor valproate induced a coordinate increase in the levels of H3K9ac and H3K4me3 at all Hoxb promoters, but not other genes, whilst H3K27me3 was unaffected. Such increases were not maintained upon removal of the inhibitor. All Hoxb genes were silent in undifferentiated ES cells, but expression was activated at defined times of differentiation in the expected 3’ to 5’ sequence. The valproate induced increase in active modifications did not induce Hoxb expression from the cluster in undifferentiated cells, nor was there any major shift in the timing of Hoxb expression in cells transiently exposed to valproate (ie. hyperacetylated) during the start of differentiation. Thus, active histone modifications at the Hox genes are uncoupled from transcription. 2009-12 2009-06 graduation eng author unchecked This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. public 201 University of Birmingham Institute of Biomedical Research d_ph FALSE na 2009-12 graduation