Mitochondrial DNA replication in pre-implantation embryonic development

45 32 110 9 159 document 110 Spikings07PhD.pdf application/pdf 20cb8dd47efb6e3229574f1ce18302d0 MD5 4740323 2011-07-19 16:12:10 http://etheses.bham.ac.uk//id/eprint/45/1/Spikings07PhD.pdf 45 1 1 application/pdf application/pdf en public

Spikings07PhD.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/23197 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/23197 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/23197 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/23198 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/23198 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/23198 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/25421 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/25421 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/25421 23198 2 48944 document 23198 preview.jpg image/jpeg 91000fca407c481c89e023901dde6fc5 MD5 12877 2013-01-29 14:39:29 http://etheses.bham.ac.uk//id/eprint/45/6/preview.jpg 45 6 6 image/jpeg image/jpeg Thumbnail Documents conversion from application/pdf to thumbnail_preview en public

preview.jpg

indexcodes.txt

small.png

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/110 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/110 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/110 archive 4031 disk0/00/00/00/45 2007-12-17 17:16:13 2019-01-23 09:51:26 2011-07-19 16:12:10 thesis show 0 Spikings Emma Catherine emma@spikings.com St. John Justin Charles J.StJohn.1@bham.ac.uk University of Birmingham Mitochondrial DNA replication in pre-implantation embryonic development unpub RG QP QH426 30sch_medi All eukaryotic cells possess mitochondrial DNA (mtDNA), which is maternally inherited through the oocyte, its replication being regulated by nuclear-encoded replication factors. It was hypothesised that mtDNA replication is highly regulated in oocytes, pre-implantation embryos and embryonic stem cells (ESCs) and that this may be disrupted following nuclear transfer (NT). MtDNA copy number decreased between 2-cell and 8-cell staged porcine embryos and increased between the morula and expanded blastocyst stages, coinciding with increased expression of mtDNA replication factors. Competent porcine oocytes replicated their mtDNA prior to and during in vitro maturation to produce and maintain the 100000 mtDNA copies required for fertilisation. Those oocytes in which mtDNA replication was delayed had reduced developmental ability. Expression of pluripotency-associated genes decreased as murine ESCs differentiated into embryoid bodies, although expression of mtDNA replication factors did not increase until the stage equivalent to organogenesis. Cross-species NT embryos in which the donor cell-derived mtDNA was replicated produced decreased developmental outcomes compared to those in which no mtDNA replication took place. Disruption of the strict regulation of mtDNA replication that occurs during early embryogenesis, as is likely following NT, may therefore contribute to the reduced developmental ability of embryos produced using such techniques. 2007-07 graduation 2631828 eng author unchecked This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. The University of Birmingham public 266 University of Birmingham Division of Medical Sciences d_ph other 2007-07 graduation