The role of extra and intra vascular cells and their molecules in modulating glomerular inflammation during health and disease

3363 17 19862 6 37850 document 19862 Kuravi12PhD.pdf application/pdf 2a337fdb6c1cb03ce9d1217062269fdb MD5 5006586 2012-05-29 11:02:31 http://etheses.bham.ac.uk//id/eprint/3363/1/Kuravi12PhD.pdf 3363 1 1 application/pdf application/pdf en public

Kuravi12PhD.pdf

accepted http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/19866 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/19866 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/19866 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/19867 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/19867 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/19867 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/19868 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/19868 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/19868 19863 5 37853 document 19863 Decl_IS_Kuravi12PhD.pdf application/pdf 3af46fc5dbe966a56afee78eb1aa93b1 MD5 27778 2012-05-29 11:02:58 http://etheses.bham.ac.uk//id/eprint/3363/2/Decl_IS_Kuravi12PhD.pdf 3363 2 2 application/pdf application/pdf en staffonly

Decl_IS_Kuravi12PhD.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/19864 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/19864 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/19864 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/19865 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/19865 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/19865 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/19869 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/19869 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/19869 19864 2 37863 document 19864 small.png image/png 166f15b19eef395a80d77933269ea3d6 MD5 10316 2012-05-29 11:19:03 http://etheses.bham.ac.uk//id/eprint/3363/3/small.png 3363 3 3 image/png thumbnail_small Thumbnail Documents conversion from application/pdf to thumbnail_small en staffonly

small.png

preview.jpg

small.png

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/19862 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/19862 http://eprints.org/relation/issmallThumbnailVersionOf http://etheses.bham.ac.uk/id/document/19862 19867 2 37866 document 19867 preview.jpg image/jpeg 1902b122baecdff49ba9aefd805ab138 MD5 22814 2012-05-29 11:19:04 http://etheses.bham.ac.uk//id/eprint/3363/6/preview.jpg 3363 6 6 image/jpeg image/jpeg Thumbnail Documents conversion from application/pdf to thumbnail_preview en staffonly

preview.jpg

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/19862 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/19862 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/19862 19868 2 37867 document 19868 indexcodes.txt text/plain d38584220e16d65ed97d940ba7815078 MD5 43382 2012-05-29 11:19:06 http://etheses.bham.ac.uk//id/eprint/3363/7/indexcodes.txt 3363 7 7 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en staffonly

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/19862 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/19862 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/19862 19869 2 37868 document 19869 indexcodes.txt text/plain 1317895ab73839c0f744f55cfc441a6a MD5 1526 2012-05-29 11:19:06 http://etheses.bham.ac.uk//id/eprint/3363/8/indexcodes.txt 3363 8 8 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en staffonly

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/19863 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/19863 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/19863 archive 2795 disk0/00/00/33/63 2012-05-29 11:24:28 2019-01-23 09:56:07 2012-05-29 11:24:28 thesis show 0 Kuravi Sahithi Jyothsna Savage Caroline Rainger Ed Tull Samantha University of Birmingham The role of extra and intra vascular cells and their molecules in modulating glomerular inflammation during health and disease unpub QR R1 10col_mede Inflammation, Antineutrophil cytoplasmic autoantibodies, podocytes, glomerular endothelial cells, neutrophils, Interleukin-6 Endothelium forms an interface between vasculature and cellular sub endothelial environment responding to the changes in the immediate environment in health and disease. During vasculitic glomerulonephritis (VGN), autoantibodies raised against neutrophil serine proteases (SP) activate the neutrophils resulting in the release of neutrophilic products onto the endothelial surface changing its ability to support neutrophil recruitment and leading to its damage. We have hypothesized that endothelial functions are modulated by intra and extracellular environments of the vasculature and that the glomerular epithelial cells (podocytes) regulate glomerular endothelial cell (GEnC) functions. This work investigated the effects of SP (Proteinase-3 (PR3) and human neutrophil elastase (HNE)) in mediating neutrophil recruitment on EnC prior to the development of injury in a concentration and time dependent fashion. Most of the effects were inhibitable by the presence of α1-antitrypsin (α1-AT). Further, using an in vitro static co-culture system, this work demonstrates an endogenous mechanism by which podocytes modulate endothelial response to inflammatory stimuli in the glomerulus under healthy and disease conditions. Overall, these studies elucidate that GEnC presents an adhesive phenotype attracting neutrophils under the influence of inflammatory cytokines. During disease conditions as in VGN, exposure of endothelium to SPs results in a pre-activation stage leading to damage with high and/or prolonged exposures. Podocytes modulate glomerular endothelial responses to cytokine stimulation in health and disease. Thus, endothelial functions are tightly regulated by interacting cells and their molecules and could be an important phenomenon in the disease process of VGN where neutrophil recruitment plays a central pathogenic role. 2012-07 graduation eng author unchecked This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. public 242 University of Birmingham School of Immunity and Infection d_ph na 2012-07 graduation