Tamoxifen resistance in breast cancer: a proteomic approach

3270 19 19185 6 36054 document 19185 Phillips12PhD.pdf application/pdf 3a792fea511175844e720560a2b040f3 MD5 5287062 2012-04-27 10:56:45 http://etheses.bham.ac.uk//id/eprint/3270/1/Phillips12PhD.pdf 3270 1 1 application/pdf application/pdf en public

Phillips12PhD.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/19189 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/19189 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/19189 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/19190 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/19190 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/19190 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/19191 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/19191 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/19191 19186 5 36057 document 19186 Decl_IS_Phillips12PhD.pdf application/pdf 206ce2d01d8012bcc9eba7cab53b334c MD5 98229 2012-04-27 10:57:04 http://etheses.bham.ac.uk//id/eprint/3270/2/Decl_IS_Phillips12PhD.pdf 3270 2 2 application/pdf application/pdf en staffonly

Decl_IS_Phillips12PhD.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/19187 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/19187 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/19187 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/19188 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/19188 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/19188 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/19192 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/19192 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/19192 19187 2 36067 document 19187 small.png image/png 7b447d0ac9d5abe1e0d42ca6ca3691b3 MD5 10340 2012-04-27 11:04:18 http://etheses.bham.ac.uk//id/eprint/3270/3/small.png 3270 3 3 image/png thumbnail_small Thumbnail Documents conversion from application/pdf to thumbnail_small en staffonly

small.png

preview.jpg

small.png

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/19185 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/19185 http://eprints.org/relation/issmallThumbnailVersionOf http://etheses.bham.ac.uk/id/document/19185 19190 2 36070 document 19190 preview.jpg image/jpeg abb73b4f2302662879df422f47d31377 MD5 11652 2012-04-27 11:04:19 http://etheses.bham.ac.uk//id/eprint/3270/6/preview.jpg 3270 6 6 image/jpeg image/jpeg Thumbnail Documents conversion from application/pdf to thumbnail_preview en staffonly

preview.jpg

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/19185 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/19185 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/19185 19191 2 36071 document 19191 indexcodes.txt text/plain 1caaadd6de3ac57668e14401352cbf57 MD5 65970 2012-04-27 11:04:23 http://etheses.bham.ac.uk//id/eprint/3270/7/indexcodes.txt 3270 7 7 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en staffonly

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/19185 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/19185 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/19185 19192 2 36072 document 19192 indexcodes.txt text/plain a38ac4d206647d749bbb5d994a0b2e86 MD5 1530 2012-04-27 11:04:23 http://etheses.bham.ac.uk//id/eprint/3270/8/indexcodes.txt 3270 8 8 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en staffonly

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/19186 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/19186 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/19186 archive 4031 disk0/00/00/32/70 2012-04-27 12:04:15 2019-01-23 09:55:54 2012-04-27 12:04:15 thesis show 0 Phillips Elisabeth Assender Jean Martin Ashley University of Birmingham Tamoxifen resistance in breast cancer: a proteomic approach unpub QH301 QR RC0254 RM 10col_mede Tamoxifen is an effective and well-tolerated treatment for early disease and/or pre-menopausal patients with breast cancer (BC); although many women go on to develop resistance. Currently the five-year survival rate following Tamoxifen resistance (TR) is < 20%; hence the mechanisms need to be better understood. Recent research has focussed on specific pathways, however additional mechanisms are involved and we investigated these using cell line models of BC (MCF7) and TR using a variety of proteomic approaches. Differential expression and phosphorylation of proteins between the MCF7 and TR cell lines were detected by antibody arrays; which detected changes in Mitogen Activated Protein Kinases and Receptor Tyrosine Kinases family members, and in apoptosis related proteins. There were 21 novel proteins found to be altered in TR. 262 quantifiable proteins were found using SILAC; 29% over expressed in resistance and 25% down regulated. 5 were subsequently picked for validation by Western blot and 2 of these (IQGAP1 and cortactin) were chosen for further investigation with siRNA and functional assays. IQGAP1 was found to play a role in TR; as decreasing expression of IQGAP1 using SiRNA decreased the proliferation of TR cells and significantly modulated the TR cells ability to invade matrigel. 2012-07 2011-09 graduation eng author unchecked This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. The University of Birmingham public 277 University of Birmingham Institute of Cancer Studies d_ph other 2012-07 graduation