Investigating the role of the cytoskeleton and signalling in the self-incompatibility response of Papaver rhoeas

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http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/780 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/780 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/780 archive 4031 disk0/00/00/03/22 2009-11-09 17:32:13 2019-01-23 09:51:55 2011-07-19 16:15:50 thesis show 0 Poulter Natalie Sarah Franklin-Tong Veronica University of Birmingham Investigating the role of the cytoskeleton and signalling in the self-incompatibility response of Papaver rhoeas unpub QK 10col_life Self-incompatibility, pollen, actin cytoskeleton, microtubule cytoskeleton, programmed cell death Appendix II is in two separate html files Many flowering plants are hermaphrodite, which poses the problem of self-fertilisation and the subsequent loss of genetic fitness in the offspring. To prevent this, plants have developed a genetically controlled mechanism called self-incompatibility (SI) which allows self (incompatible) pollen to be recognised and rejected before fertilisation can occur. The SI response of Papaver rhoeas (field poppy) has been extensively studied at the molecular and cellular level. Rejection of incompatible pollen occurs on the stigma surface when the pollen S-determinant PrpS, a transmembrane protein, interacts with the stigmatic S-determinant, secreted S-proteins. This triggers a calcium-mediated signalling cascade that targets the cytoskeleton and results in programmed cell death (PCD) of incompatible pollen. Work presented in this thesis investigated the localisation of PrpS and S-proteins. Other studies investigated the role of the cytoskeleton in SI. These demonstrated the involvement of the microtubule cytoskeleton for the first time. Microtubules were rapidly depolymerised and this was implicated in signalling to PCD. The actin cytoskeleton has previously been shown to exhibit biphasic alterations during SI involving depolymerisation, followed by formation of F-actin foci. Studies described here represent the first steps toward characterisation of the F-actin foci. Their potential involvement in PCD and signalling is discussed. 2009-12 2009-06 graduation eng author unchecked This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. public 267 University of Birmingham School of Biosciences d_ph na 2009-12 graduation