Tissue engineering a ligamentous construct

2989 21 16403 6 28129 document 16403 Mehrban11PhD.pdf application/pdf 51f2d6888c22eb970a609e374e67d41f MD5 8194347 2011-11-22 14:36:51 http://etheses.bham.ac.uk//id/eprint/2989/1/Mehrban11PhD.pdf 2989 1 1 application/pdf application/pdf en public

Mehrban11PhD.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/16407 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/16407 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/16407 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/16408 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/16408 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/16408 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/16409 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/16409 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/16409 16404 5 28132 document 16404 Decl_IS_Mehrban11PhD.pdf application/pdf f35c34270ff86e24af79f09889a36759 MD5 97581 2011-11-22 14:37:11 http://etheses.bham.ac.uk//id/eprint/2989/2/Decl_IS_Mehrban11PhD.pdf 2989 2 2 application/pdf application/pdf en staffonly

Decl_IS_Mehrban11PhD.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/16405 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/16405 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/16405 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/16406 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/16406 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/16406 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/16410 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/16410 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/16410 16405 2 28141 document 16405 small.png image/png ba8100c7879a7155de991d972287e778 MD5 10327 2011-11-22 14:45:45 http://etheses.bham.ac.uk//id/eprint/2989/3/small.png 2989 3 3 image/png thumbnail_small Thumbnail Documents conversion from application/pdf to thumbnail_small en staffonly

small.png

preview.jpg

small.png

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/16403 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/16403 http://eprints.org/relation/issmallThumbnailVersionOf http://etheses.bham.ac.uk/id/document/16403 16408 2 28144 document 16408 preview.jpg image/jpeg 35765f9abc6ae43eb7c2bd6155bc3412 MD5 12262 2011-11-22 14:45:46 http://etheses.bham.ac.uk//id/eprint/2989/6/preview.jpg 2989 6 6 image/jpeg image/jpeg Thumbnail Documents conversion from application/pdf to thumbnail_preview en staffonly

preview.jpg

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/16403 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/16403 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/16403 16409 2 28145 document 16409 indexcodes.txt text/plain bd27b279be50ee9eff614da450238db7 MD5 53020 2011-11-22 14:45:48 http://etheses.bham.ac.uk//id/eprint/2989/7/indexcodes.txt 2989 7 7 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en staffonly

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/16403 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/16403 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/16403 16410 2 28146 document 16410 indexcodes.txt text/plain b7801bf6387d2bc098ca020eceb279e6 MD5 1501 2011-11-22 14:45:48 http://etheses.bham.ac.uk//id/eprint/2989/8/indexcodes.txt 2989 8 8 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en staffonly

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/16404 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/16404 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/16404 archive 4031 disk0/00/00/29/89 2011-11-22 15:16:22 2019-01-23 09:55:16 2011-11-22 15:16:22 thesis show 0 Mehrban Nazia Grover Liam University of Birmingham Tissue engineering a ligamentous construct unpub QM QP RC1200 TP 10col_ephy Tendon and ligament damage causes extreme pain and decreased joint functionality. Current repair methods cannot restore original joint biomechanics nor promote regeneration of native tissue. Recent advances in tendon and ligament repair have involved engineering tissue using cell-seeded scaffolds. Self-aligned cellular structures, similar to those in ligaments and tendons, have been successfully formed, albeit with weak attachment between construct and bone. Calcium phosphates form an intimate bond with both soft and hard tissues and have successfully been used in tissue engineering bone, whilst hydrogels have often been used as cellular scaffolds. This thesis explores agarose, gelatin, carrageenan and fibrin hydrogels as potential soft tissue scaffolds. Fibrin gel exhibited high cellular compatibility with highest metabolic activity on day 14. Although the cellular gel contracted significantly, it was found that the dry weight remained stable in both the acellular and cellular forms. 3D powder printed calcium phosphate scaffolds remained structurally stable after immersion in cell culture media with immersion in protein-rich sera promoting tenocyte attachment. Bracket designs were developed to enhance grip of the cell-seeded fibrin. Ligament constructs were selfsupporting and exhibited structural characteristics similar to native connective tissue. Tenocyte density peaked on day 14, with added L-proline and ascorbic acid inducing a constant level of glycosaminoglycans and 7.4 ± 1.5 % w/w collagen. This research may significantly enhance the clinical application of tissue engineered ligaments and tendons. 2011-12 graduation eng author unchecked This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. Advantage West Midlands public 310 University of Birmingham School of Chemical Engineering d_ph other bbsrc european_commission 2011-12 graduation