The role of CCRL2 in the regulation of germinal centre B-cell migration and a new regulatory step in T-cell migration into tissue during inflammation; separating the wanted from the unwanted

2936 16 16790 6 29203 document 16790 Cook_11_MRes.pdf application/pdf f86a63c5b2f7845f8635bf40c8e2b5a7 MD5 3042415 2011-11-25 14:41:20 http://etheses.bham.ac.uk//id/eprint/2936/1/Cook_11_MRes.pdf 2936 1 1 application/pdf application/pdf en public

Cook_11_MRes.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/16794 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/16794 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/16794 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/16795 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/16795 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/16795 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/16796 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/16796 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/16796 16791 5 29207 document 16791 Decl_IS_Cook_11_MRes.pdf application/pdf b1852c69ce66eb2b09317e9c21c853f9 MD5 370888 2011-11-25 14:43:37 http://etheses.bham.ac.uk//id/eprint/2936/2/Decl_IS_Cook_11_MRes.pdf 2936 2 2 application/pdf application/pdf en staffonly

Decl_IS_Cook_11_MRes.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/16792 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/16792 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/16792 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/16793 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/16793 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/16793 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/16797 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/16797 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/16797 16792 2 29212 document 16792 small.png image/png 4cd92081db78a9ce01ef4e4ce47ef139 MD5 7717 2011-11-25 14:44:10 http://etheses.bham.ac.uk//id/eprint/2936/3/small.png 2936 3 3 image/png thumbnail_small Thumbnail Documents conversion from application/pdf to thumbnail_small en staffonly

small.png

preview.jpg

small.png

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/16790 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/16790 http://eprints.org/relation/issmallThumbnailVersionOf http://etheses.bham.ac.uk/id/document/16790 16795 2 29215 document 16795 preview.jpg image/jpeg a51ba2d5dd1157930ce4c3397456a6f1 MD5 17653 2011-11-25 14:44:11 http://etheses.bham.ac.uk//id/eprint/2936/6/preview.jpg 2936 6 6 image/jpeg image/jpeg Thumbnail Documents conversion from application/pdf to thumbnail_preview en staffonly

preview.jpg

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/16790 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/16790 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/16790 16796 2 29216 document 16796 indexcodes.txt text/plain d70b9375ff96b8ee547adef3af448919 MD5 30141 2011-11-25 14:44:12 http://etheses.bham.ac.uk//id/eprint/2936/7/indexcodes.txt 2936 7 7 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en staffonly

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/16790 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/16790 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/16790 16797 2 29217 document 16797 indexcodes.txt text/plain 9f92e20ce787f144dee26ba8145d449d MD5 3064 2011-11-25 14:44:12 http://etheses.bham.ac.uk//id/eprint/2936/8/indexcodes.txt 2936 8 8 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en staffonly

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/16791 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/16791 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/16791 archive 4031 disk0/00/00/29/36 2011-11-25 14:43:58 2019-01-23 09:55:09 2011-11-25 14:43:58 thesis show 0 Cook Sarah Louise University of Birmingham The role of CCRL2 in the regulation of germinal centre B-cell migration and a new regulatory step in T-cell migration into tissue during inflammation; separating the wanted from the unwanted unpub RA RC 10col_mede CC-Chemokine receptor like 2 (CCRL2) is the newest member of the atypical chemokine receptor family, a set of proteins which tend to act as “decoy receptors”, causing a chemoattractant gradient of their ligands. Using SYBR-Green and Taqman RT-PCR analysis of murine tissue, this study aimed to characterise the expression of CCRL2 in the spleen, thymus and lymph nodes. Using specifically designed primers, the PCR techniques detected an alternative, “long”, spliced variant of CCRL2 within the thymus. CCRL2 mRNA expression was also defined over 8 days, during the lifetime of the germinal centre. CCRL2 mRNA expression was measured within B cells, the germinal centre or the T Zone. Due to its high expression within plasma B cells at days 4 and 7, CCRL2 may be involved in plasma cell exit from the GC. However, CCRL2 mRNA was also expressed within the germinal centre on days 7 and 8, which may suggest the receptor also has a role in germinal centre breakdown. Finally, this study also started the process of monoclonal antibody production to murine CCRL2. CCRL2 DNA was successfully cloned into a plasmid vector and transformed into E. coli in preparation for transfection into a mammalian cell line 2011-12 2011-08 graduation eng This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. public 120 University of Birmingham School of Immunity and Infection m_rs wellcome_trust 2011-12 graduation