Cell biology of settlement and adhesion processes of biofouling algae

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http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/524 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/524 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/524 archive 4031 disk0/00/00/01/94 2009-01-06 15:20:26 2019-01-23 09:51:41 2011-07-19 16:14:01 thesis show 0 Thompson Stephanie Eleanor Mary s.e.y.thompson@bham.ac.uk Callow J. A. Callow Maureen Brownlee Colin University of Birmingham Marine Biological Association of the United Kingdom Cell biology of settlement and adhesion processes of biofouling algae unpub QH301 30sch_bios adhesion, amperometry, calcium, DAF-FM, diatom, endocytosis, exocytosis, Ulva The aim of the research presented in this thesis was to investigate the cell biology behind the settlement and adhesion processes of biofouling algae. Using the fluorescent dye FM 1-43 in Ulva zoospores to follow membrane recycling, rapid mass membrane retrieval of FM 1-43-labelled plasma membrane was found to occur to an endosomal compartment during settlement. Biolistic delivery of dextran Oregon Green BAPTA-1 and Texas Red enabled ratiometric imaging with a 5-fold greater response to Ca\(^{2+}\)-ionophores than AM-ester Ca\(^{2+}\) indicators. During settlement, zoospores exhibited both localised and diffuse increases in cytosolic calcium implying a role in secretion of the adhesive. Secretion of redox-active substrates was detected using amperometry when settled spores were mechano-stimulated. Secretory events were similar to those seen in bovine chromaffin cells with the presence of foot signals in the recordings implying a role for a ‘fusion pore’ in exocytosis. Using DAF-FM DA nitric oxide (NO) production in Seminavis robusta was found to be 4-fold greater on a surface to which the cells adhered weakly than on a surface to which they attached more strongly. Increased NO reduced attachment strength and it is thought that NO may play a signalling and/or regulatory role in diatom adhesion. 2008-07 graduation 2726586 eng author unchecked This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. public 215 University of Birmingham School of Biosciences d_ph FALSE bbsrc 2008-07 graduation