The role of platelets and their associated recruitment mechanisms, in intestinal ischaemia reperfusion injury

1489 27 3518 7 5080 document 3518 Holyer_11_PhD.pdf application/pdf 64398ca9585163fc4a8cad47dea3ccd5 MD5 6819934 2011-07-19 16:37:12 http://etheses.bham.ac.uk//id/eprint/1489/1/Holyer_11_PhD.pdf 1489 1 1 application/pdf application/pdf en public

Holyer_11_PhD.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/5322 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/5322 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/5322 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/5323 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/5323 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/5323 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/5326 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/5326 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/5326 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/14222 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/14222 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/14222 3519 6 5081 document 3519 Decl_IS_Holyer_11_PhD.pdf application/pdf 9bc8748ea57b7171552d509786da085f MD5 277233 2011-07-19 16:37:13 http://etheses.bham.ac.uk//id/eprint/1489/2/Decl_IS_Holyer_11_PhD.pdf 1489 2 2 application/pdf application/pdf en staffonly

Decl_IS_Holyer_11_PhD.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/5324 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/5324 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/5324 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/5325 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/5325 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/5325 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/5327 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/5327 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/5327 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/14223 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/14223 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/14223 5322 2 7301 document 5322 small.png image/png 1c380abb7ec67ba2b4cc43d3b7d6aff4 MD5 3789 2011-07-19 17:55:50 http://etheses.bham.ac.uk//id/eprint/1489/3/small.png 1489 3 3 image/png thumbnail_small Thumbnail Documents conversion from application/pdf to thumbnail_small en public

small.png

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/3518 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/3518 http://eprints.org/relation/issmallThumbnailVersionOf http://etheses.bham.ac.uk/id/document/3518 5323 2 7302 document 5323 preview.jpg image/jpeg 4fd05f748324b93cbc24fe69a1584e57 MD5 13542 2011-07-19 17:55:51 http://etheses.bham.ac.uk//id/eprint/1489/4/preview.jpg 1489 4 4 image/jpeg image/jpeg Thumbnail Documents conversion from application/pdf to thumbnail_preview en public

preview.jpg

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/3518 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/3518 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/3518 5324 2 7304 document 5324 small.png image/png 5f8f1d2bcc83681c1cf4acb185ac306f MD5 7532 2011-07-19 17:55:51 http://etheses.bham.ac.uk//id/eprint/1489/5/small.png 1489 5 5 image/png thumbnail_small Thumbnail Documents conversion from application/pdf to thumbnail_small en staffonly

small.png

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/3519 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/3519 http://eprints.org/relation/issmallThumbnailVersionOf http://etheses.bham.ac.uk/id/document/3519 5325 2 7305 document 5325 preview.jpg image/jpeg f7d975dee517e339b09204517b648305 MD5 28567 2011-07-19 17:55:52 http://etheses.bham.ac.uk//id/eprint/1489/6/preview.jpg 1489 6 6 image/jpeg image/jpeg Thumbnail Documents conversion from application/pdf to thumbnail_preview en staffonly

preview.jpg

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/3519 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/3519 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/3519 5326 2 7306 document 5326 indexcodes.txt text/plain d1be1bcc3b6f72ee9e61115a8c43537d MD5 33243 2011-07-19 17:55:55 http://etheses.bham.ac.uk//id/eprint/1489/7/indexcodes.txt 1489 7 7 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en public

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/3518 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/3518 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/3518 5327 2 7307 document 5327 indexcodes.txt text/plain cfa255f80612ec0840162fcb9e61f75d MD5 3079 2011-07-19 17:55:55 http://etheses.bham.ac.uk//id/eprint/1489/8/indexcodes.txt 1489 8 8 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en staffonly

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/3519 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/3519 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/3519 14222 2 21953 document 14222 preview.png image/png 3132ccd7ff33129a5790a304072b6f8e MD5 17014 2011-07-21 10:56:06 http://etheses.bham.ac.uk//id/eprint/1489/9/preview.png 1489 9 9 image/png image/png en public

preview.png

http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/3518 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/3518 http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/3518 14223 2 21956 document 14223 preview.png image/png 7a5793564bc2d0a63b092317d20e1d88 MD5 30505 2011-07-21 10:56:06 http://etheses.bham.ac.uk//id/eprint/1489/10/preview.png 1489 10 10 image/png image/png en staffonly

preview.png

http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/3519 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/3519 http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/3519 archive 4031 disk0/00/00/14/89 2011-04-04 13:02:17 2019-01-22 11:53:17 2011-07-19 16:37:12 thesis show 0 Holyer Ian Watson Steve P. Kalid Neena University of Birmingham The role of platelets and their associated recruitment mechanisms, in intestinal ischaemia reperfusion injury unpub RB RC 10col_mede Recent studies have demonstrated a key role for platelets and microthrombi in the pathophysiology of intestinal ischaemia-reperfusion (IR) injury. The research described in this thesis investigates the role of the major platelet glycoprotein receptors in recruiting and activating platelets following intestinal IR injury, namely GPVI, GPIb-IX-V and the integrins IIb3 and 21. Intravital microscopy was utilised to monitor individual platelet adhesion and microthrombus formation in anaesthetised mice undergoing intestinal IR injury \(in\) \(vivo\) using a novel dual labelling methodology. This study focussed on the microcirculation of the mucosal villi as this luminal surface is most susceptible to IR injury. We demonstrate that it was necessary to inhibit both microthrombus formation as well as platelet-leukocyte-endothelial interactions in order to ensure longer lasting improvement in gut microcirculation and histology. This was achieved through a dual therapy that targeted both GPIb and P-selectin. The strongest anti-platelet effect was observed with blocking the IIb3 integrin, but this was also associated with a sustained bleeding from the mucosal surface. Overall, the research within this thesis suggests that therapeutic strategies targeting GPIb and P-selectin may prove beneficial in improving the clinical morbidity associated with gut IR injury. 2011 2010-09 graduation eng This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. public 233 University of Birmingham School of Clinical and Experimental Medicine d_ph na 2011 graduation