Identifying biosynthetic pathways for mycobacterial cell wall components using transposon mutagenesis

1140 26 2914 7 4170 document 2914 Chen_10_PhD.pdf application/pdf 854801874256e87fd5d000bb78955fcd MD5 1991645 2011-07-19 16:30:23 http://etheses.bham.ac.uk//id/eprint/1140/1/Chen_10_PhD.pdf 1140 1 1 application/pdf application/pdf en public

Chen_10_PhD.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/6833 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/6833 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/6833 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/6834 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/6834 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/6834 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/6837 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/6837 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/6837 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/13759 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/13759 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/13759 2916 6 4172 document 2916 Decl_IS_Chen_10_PhD.pdf application/pdf ea1081e9ddda7820a0708b126e402d9c MD5 40904 2011-07-19 16:30:24 http://etheses.bham.ac.uk//id/eprint/1140/2/Decl_IS_Chen_10_PhD.pdf 1140 2 2 application/pdf application/pdf en staffonly

Decl_IS_Chen_10_PhD.pdf

http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/6835 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/6835 http://eprints.org/relation/hassmallThumbnailVersion http://etheses.bham.ac.uk/id/document/6835 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/6836 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/6836 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/6836 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/6838 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/6838 http://eprints.org/relation/hasIndexCodesVersion http://etheses.bham.ac.uk/id/document/6838 http://eprints.org/relation/hasVolatileVersion http://etheses.bham.ac.uk/id/document/13760 http://eprints.org/relation/haspreviewThumbnailVersion http://etheses.bham.ac.uk/id/document/13760 http://eprints.org/relation/hasVersion http://etheses.bham.ac.uk/id/document/13760 6833 2 9037 document 6833 small.png image/png e4245c6148df123ab9a29b0fef832aae MD5 3351 2011-07-19 19:50:49 http://etheses.bham.ac.uk//id/eprint/1140/3/small.png 1140 3 3 image/png thumbnail_small Thumbnail Documents conversion from application/pdf to thumbnail_small en public

small.png

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/2914 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/2914 http://eprints.org/relation/issmallThumbnailVersionOf http://etheses.bham.ac.uk/id/document/2914 6834 2 9038 document 6834 preview.jpg image/jpeg 745d337c6000927fb9c3a8cac4cc34ef MD5 13152 2011-07-19 19:50:50 http://etheses.bham.ac.uk//id/eprint/1140/4/preview.jpg 1140 4 4 image/jpeg image/jpeg Thumbnail Documents conversion from application/pdf to thumbnail_preview en public

preview.jpg

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/2914 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/2914 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/2914 6835 2 9040 document 6835 small.png image/png 14ff801fadc61ebfbc33abb39084e560 MD5 7262 2011-07-19 19:50:52 http://etheses.bham.ac.uk//id/eprint/1140/5/small.png 1140 5 5 image/png thumbnail_small Thumbnail Documents conversion from application/pdf to thumbnail_small en staffonly

small.png

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/2916 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/2916 http://eprints.org/relation/issmallThumbnailVersionOf http://etheses.bham.ac.uk/id/document/2916 6836 2 9041 document 6836 preview.jpg image/jpeg 818a830ecd8aa917bca4c16d5f62e363 MD5 28736 2011-07-19 19:50:54 http://etheses.bham.ac.uk//id/eprint/1140/6/preview.jpg 1140 6 6 image/jpeg image/jpeg Thumbnail Documents conversion from application/pdf to thumbnail_preview en staffonly

preview.jpg

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/2916 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/2916 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/2916 6837 2 9042 document 6837 indexcodes.txt text/plain e33a22afcafbc5e3695410e4e0324e71 MD5 40605 2011-07-19 19:50:57 http://etheses.bham.ac.uk//id/eprint/1140/7/indexcodes.txt 1140 7 7 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en public

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/2914 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/2914 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/2914 6838 2 9043 document 6838 indexcodes.txt text/plain 897651de672cd170a35ec43818711be3 MD5 2915 2011-07-19 19:50:57 http://etheses.bham.ac.uk//id/eprint/1140/8/indexcodes.txt 1140 8 8 text/plain indexcodes Generate index codes conversion from application/pdf to indexcodes en staffonly

indexcodes.txt

http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/2916 http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/2916 http://eprints.org/relation/isIndexCodesVersionOf http://etheses.bham.ac.uk/id/document/2916 13759 2 20564 document 13759 preview.png image/png 86df2d4e9a7172ed34d6fe7dbbef1048 MD5 10860 2011-07-21 10:52:48 http://etheses.bham.ac.uk//id/eprint/1140/9/preview.png 1140 9 9 image/png image/png en public

preview.png

http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/2914 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/2914 http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/2914 13760 2 20567 document 13760 preview.png image/png 5a2cec98e71ae2fed5b60918567ed66c MD5 28348 2011-07-21 10:52:49 http://etheses.bham.ac.uk//id/eprint/1140/10/preview.png 1140 10 10 image/png image/png en staffonly

preview.png

http://eprints.org/relation/isVolatileVersionOf http://etheses.bham.ac.uk/id/document/2916 http://eprints.org/relation/ispreviewThumbnailVersionOf http://etheses.bham.ac.uk/id/document/2916 http://eprints.org/relation/isVersionOf http://etheses.bham.ac.uk/id/document/2916 archive 4031 disk0/00/00/11/40 2010-11-25 13:05:13 2019-01-23 09:53:34 2011-07-19 16:30:23 thesis show 0 Chen Jiemin Besra Gurdyal S. Bhatt Apoorva University of Birmingham Identifying biosynthetic pathways for mycobacterial cell wall components using transposon mutagenesis unpub QR 10col_life Mycobacterium tuberculosis, the causative agent of the infectious disease tuberculosis, has a distinct lipid-rich cell wall. Several anti-TB drugs target cell wall biosynthetic pathways. A good understanding of its biosynthesis will provide helpful clues for the development of novel drug targets. A strategy based on random transposon (Tn) mutagenesis with two different screening criteria, altered colony morphology and mycobacteriophage resistance, was developed. Non-pathogenic Mycobacterium smegmatis and the fish pathogen Mycobacterium marinum were used for generating Tn-mutant libraries. From the colony morphology screen, two out of eight genes identified from M. smegmatis Tn-mutants and eleven out of twenty from M. marinum Tn-mutants with altered colony morphology were directly involved in cell wall synthesis. One mutant from each species was chosen for further study, the M. smegmatis 3D9 Tn-mutant with the only isocitrate dehydrogenase (icd) gene disrupted and the M. marinum 8G10 mutant with a Tn inserted into the promoter region of MMAR0978 with a deficiency in methoxymycolates production. Four mutants resistant to generalised transducing phage I3 were identified with a Tn insertion in a gene cluster involved in the biosynthesis of the cell wall associated glycopeptidolipids (GPLs), demonstrating the potential of using phage-resistant mutants for identifying cell wall biosynthetic genes. 2010-12 2010-09 graduation eng This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. The Darwin Trust of Edinburgh public 186 University of Birmingham School of Biosciences d_ph other 2010-12 graduation