Smart adsorption materials and systems for recovery of high value protein products

Cao, Ping (2016). Smart adsorption materials and systems for recovery of high value protein products. University of Birmingham. Ph.D.

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In this context, we produced thermoresponsive ionic exchange adsorbents via 'grafting from' polymerisation, and consequently applied them to a novel travelling cooling zone reactor (TCZR). Sepharose CL-6B, Superose 6 prep grade, and Superose 12 prep grade were selected as the base matrices in order to find the most suitable
particle size and pore diameter for the modification with thermoresponsive polymer. Both cation exchange adsorbents (thermoCEX) and anionic exchange adsorbents (thermoAEX) modified with poly(Nisopropylacrylamide) or pNIPAAm were introduced in Chapter 2, 3, and 5. All resins' binding affinity and maximum adsorption capacity increased with elevated temperature, however, the protein 'adsorption-desorption' behaviour of thermoCEX is much superior to that of thermoAEX. We also introduced cation exchange adsorbents with poly( ethylene glycol) or PEG in Chapter 4, although the thennoresponsiveness of thermoCEX-PEG-S6PG has been found even better than those of thermoCEX, the dynamic protein binding performance is limited due to its relatively lower protein binding capacity at higher temperature.

Type of Work: Thesis (Doctorates > Ph.D.)
Award Type: Doctorates > Ph.D.
College/Faculty: Colleges (2008 onwards) > College of Engineering & Physical Sciences
School or Department: School of Chemical Engineering
Funders: None/not applicable
Subjects: T Technology > TP Chemical technology


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