Development of fluorophore-tagged DNA probes for cellular imaging applications

Bamford, Rosemary Anne (2015). Development of fluorophore-tagged DNA probes for cellular imaging applications. University of Birmingham. Ph.D.

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Abstract

Single nucleotide polymorphisms (SNPs) are single base variations in DNA which give genetic variation. However, SNPs can also be linked to the development of certain diseases. Modified oligonucleotides used to probe biological changes and processes have become an important focus of scientific research. Fluorescent tagging of DNA can be used to sense SNPs in DNA targets through differences in emission intensity on the formation of a duplex.
An anthracene-tagged DNA probe developed by Tucker $$et$$ $$al$$. is able to discriminate between a fully complementary DNA target sequence and one with a single base difference. This thesis describes how SNP sensing with anthracene-tagged DNA has been extended to SNPs in RNA targets and sequences associated with Alzheimer's disease. Finally, a new dual fluorophore DNA probe was designed for SNP sensing via FRET.

Type of Work: Thesis (Doctorates > Ph.D.)
Award Type: Doctorates > Ph.D.
Supervisor(s):
Supervisor(s)EmailORCID
Tucker, JamesUNSPECIFIEDUNSPECIFIED
Wilkie Professor, JohnUNSPECIFIEDUNSPECIFIED
Dehghani, HamidUNSPECIFIEDUNSPECIFIED
Licence:
College/Faculty: Colleges (2008 onwards) > College of Engineering & Physical Sciences
School or Department: School of Chemistry
Funders: None/not applicable
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH426 Genetics
URI: http://etheses.bham.ac.uk/id/eprint/6406

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