# Project and evolutionary dynamics of Pseudomonas aeruginosa during anti-adhesion therapy AND The purification and crystallisation of five Bdellovibrio proteins used during attack phase

Hardy, David (2015). Project and evolutionary dynamics of Pseudomonas aeruginosa during anti-adhesion therapy AND The purification and crystallisation of five Bdellovibrio proteins used during attack phase. University of Birmingham. M.Res.

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## Abstract

This Masters by Research thesis comprises of two different projects each conducted at the University of Birmingham between 2013 and 2014. The first project was about using an anti-adhesion FimH antagonist on $$P.$$ $$aeruginosa$$ to evaluate their population and evolutionary dynamics. This data was then used to help mathematical models on the effects of anti-adhesion molecules during and bacterial infection. Six clinical isolates were for their antibiotic resistance to meropenem and imipenem. Once a resistant and susceptible isolate was found their growth rates, killing rates, attachment amounts and phagocyte killing capacity was measured. The second project involved protein purification and crystallisation of five $$Bdellovibrio$$ proteins during attack phase. During attack phase $$Bdellovibrio$$ up regulates certain proteins and finding the structure of these proteins will help understand how the function. This could be used as way to control $$Bdellovibrio$$ and use it as at living antibiotic. $$Bdellovibrio$$ is a predatory bacterium capable of invading and replicating within Gram negative bacteria. The overall goal of this project was to purify five $$Bdellovibrio$$ proteins to a high enough purity and concentration to create protein crystals. The crystals would then be diffracted through x-ray crystallography to deduce the molecular structure.

Type of Work: Thesis (Masters by Research > M.Res.)
Award Type: Masters by Research > M.Res.
Supervisor(s):
Supervisor(s)EmailORCID
Krachler, Anne-­MarieUNSPECIFIEDUNSPECIFIED
Lovering, AndrewUNSPECIFIEDUNSPECIFIED
Licence:
College/Faculty: Colleges (2008 onwards) > College of Life & Environmental Sciences
School or Department: School of Biosciences
Funders: None/not applicable
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology > QR355 Virology
URI: http://etheses.bham.ac.uk/id/eprint/5835

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