Trzcińska, Zuzanna
(2014).
Development of peptide sequences which are able to bind to the surface of titanium and hydroxyapatite.
University of Birmingham.
M.Res.
Abstract
Titanium implants are commonly used in the replacement of load bearing joints. Poor and slow initial integration of the titanium with the natural bone tissue is the major cause of long postsurgical healing and short life of an implant. This problem can be overcome by the use of biomimetic coatings made of naturally synthesised materials. Hydroxyapatite is the major mineral component of bone tissue and when applied as an implant coating it has shown improved enhancement of the new bone tissue formation. In addition biomolecules such as peptide aptamers, which have the ability to bind electrostatically to inorganic materials in ambient conditions, are currently being researched.
In this research peptide FITC labelled peptide aptamers sequences made of titanium binding, hydroxyapatite binding motifs and hybrid peptides conjugating both sequences, were synthesized and characterized. They were then coated on Ti6Al4V and hydroxyapatite substrates. Fluorescence Microscopy have proved successful attachment of the molecules on the inorganic surfaces. Peptide release rates from the surface were measured in aqueous solutions at different temperatures and pH values, which mimic the different environments implants can be exposed. It was shown that the peptide stability in tested environment is insufficient to provide a long lasting biomimetic coating.
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