Factors affecting CD4 T cell recognition of the EBV-encoded tumour antigen EBNA1

Leonard, Henry (2014). Factors affecting CD4 T cell recognition of the EBV-encoded tumour antigen EBNA1. University of Birmingham. M.Res.

PDF - Accepted Version

Download (1MB)


Epstein-Barr virus (EBV) is associated with 200,000 cases of cancer per year. Although these include B cell lymphomas and epithelial carcinomas, the ability of T cells specific to viral antigens to target the latter, which constitute the majority of cases, has not been explored. In this study I have investigated the processing and CD4 T cell recognition of epithelial cells expressing EBNA1, a virally-encoded protein expressed in all EBV-associated malignancies. EBNA1 was equally stable in B cells and epithelial cells but EBNA1-specific CD4 T cell clones were nevertheless able to recognise both types of target cell. The potential effects of EBV-encoded micro-RNA BART18 on autophagy and CD4 T cell recognition were examined and showed that the loss of BART18 increased the autophagy levels of EBV-positive Jijoye Burkitt lymphoma cells under nutrient starvation conditions. Finally, the effects of a pharmacological inhibitor of EBNA1 dimerisation, JLP2, on the viability and T cell recognition of EBV-positive B cells was also investigated. JLP2 did not increase CD4 T cell recognition but transiently decreased the viability of one of two EBV-positive lymphoblastoid cell lines coincident with this line’s uptake of the drug.

Type of Work: Thesis (Masters by Research > M.Res.)
Award Type: Masters by Research > M.Res.
College/Faculty: Colleges (2008 onwards) > College of Medical & Dental Sciences
School or Department: Institute of Cancer Studies
Funders: None/not applicable
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
URI: http://etheses.bham.ac.uk/id/eprint/5487


Request a Correction Request a Correction
View Item View Item


Downloads per month over past year