Analysis of biological pathways, associated with orthodontic force, using multiplex arrays

Wilson, Jay Louise (2011). Analysis of biological pathways, associated with orthodontic force, using multiplex arrays. University of Birmingham. M.Phil.

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Abstract

Objective - investigate tissue changes induced by orthodontic forces at tension and compression sites. Methods - Gingival Crevicular Fluid (GCF), was collected from maxillary canines and second molars from volunteers (n=21), before appliance placement and tooth extraction (B); 3 months into orthodontic treatment (T1); at 4 hours (T2); 1 week (T3); and 6 weeks (T4) after application of a canine distalising force. Periodontal health, plaque scores, canine movement and GCF volumes were recorded. Cytokines (GM-CSF, IFN, IL-1, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, TNF), tissue (MMP-9, TIMP-1 and 2) and bone metabolism (RANKL, OPG) biomarkers were measured. Results –IL-1β, IL-8, TNFα, MMP-9 increased in canine tension sites and TIMPs 1 and 2 from T2-T4, canine compression sites showed increases in IL-1β and IL-8 only at T2, MMP-9 at T3 and T4 and RANKL at T4. GCF volume increased T2 - T4, but plaque and periodontal measures did not. Conclusions - Significant changes in the profile of several biomarkers consistant with tissue remodelling were seen. Differences between tension and compression sites were not statistically significant. TNFα, GM-CSF, IL-1β, IL-8 correlated with the rate of tooth movement. MMP-9, TIMP-1, TIMP-2 correlated with tooth movement 4 hours after the distalising force was applied.

Type of Work:

Thesis (Masters by Research > M.Phil.)

Award Type:

Masters by Research > M.Phil.

Supervisor(s):