Preparation of benzoboroxole–modified gold surfaces for selective oligosaccharide and glycoprotein recognition

Tagger, Yazmin Kaur (2021). Preparation of benzoboroxole–modified gold surfaces for selective oligosaccharide and glycoprotein recognition. University of Birmingham. Ph.D.

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Recognition of oligosaccharides is associated with very limited specificity due to their strong solvation in water and the high degree of subtle structural variations between them. Here, oligosaccharide recognition sites are created on material surfaces with unmatched, binary on–off binding behaviour, sharply discriminating a target oligosaccharide over closely related carbohydrate structures. The basis for the superselective binding behaviour relies on the highly efficient generation of a pure, high order complex of the oligosaccharide target with synthetic carbohydrate receptor sites, in which the spatial arrangement of the multiple receptors in the complex is preserved upon material surface incorporation. The synthetic binding scaffolds can be easily tailored to recognise different oligosaccharides and glycoconjugates, opening up a realm of possibilities for their use in a wide field of applications, ranging from life sciences to diagnostics.

In this thesis, a modular synthetic approach is to be created that can harness both the construction of high–yield, complex oligosaccharide–synthetic carbohydrate receptor assemblies and the precise generation of surface–confined templated binding sites. Therefore, recognition sites of unparalleled oligosaccharide discrimination are to be created.

The synthesis and characterisation of various self–assembled monolayers (SAMs) and oligosaccharide–synthetic carbohydrate receptor complexes are to be described alongside molecular imprinting studies which will determine the binding of various saccharides. Surface plasmon resonance (SPR) is to be used and from these experiments, the selectivity and sensitivity of the binding can be calculated.

Type of Work: Thesis (Doctorates > Ph.D.)
Award Type: Doctorates > Ph.D.
Licence: All rights reserved
College/Faculty: Colleges (2008 onwards) > College of Engineering & Physical Sciences
School or Department: School of Chemical Engineering
Funders: Engineering and Physical Sciences Research Council
Subjects: T Technology > TP Chemical technology


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