Single particle and single cell ICP-MS applications in nanomaterial safety assessment

Fryer, Benjamin James ORCID: 0000-0002-1466-986X (2020). Single particle and single cell ICP-MS applications in nanomaterial safety assessment. University of Birmingham. Ph.D.

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This thesis describes the development of single particle (spICP-MS) and single cell inductively coupled plasma mass spectrometry (SC-ICP-MS) analytical techniques for environmental and ecotoxicological analysis. Single particle and single cell ICP-MS protocols and application notes were developed within this thesis for a nanomaterials characterisation and interactions with living systems.

It was shown that the composition of complex multi-element nanoparticles could be determined by measuring each of the individual elements and converting particle volume to particle size, using a quadrupole based ICP-MS. The resulting particle sizes and density of multiple, multi-element nanoparticles results were comparable to other analytical techniques (DLS, DCS and TEM), but the measurement is faster and easier.

Using single particle ICP-MS, gold nanoparticles that had been ingested by isopods were analysed and quantified. A surprising result from this investigation was that irrespective of the form in which gold was presented to the isopods, i.e. as gold nanoparticles of 80 nm or ions, the gold measured in the Isopods was consistently characterised as being nanoparticle of around 60nm found within the main body and hepatopancreas of the isopods, indicating nanoparticle formation and transformation in situ.

Single cell ICP-MS was used to measure the intrinsic metal within multiple cell lines, but was found to be inconsistent or irreproducible between biological replicates, but it was possible to measure the metal concentration reliably within cells that had been spiked with a target metal.

Finally, single organism ICP-MS was developed, with the analysis of entire daphnids (daphnia magna and daphnia pulex), pushing the instrument capabilities further.

Type of Work: Thesis (Doctorates > Ph.D.)
Award Type: Doctorates > Ph.D.
Licence: All rights reserved
College/Faculty: Colleges (2008 onwards) > College of Life & Environmental Sciences
School or Department: School of Geography, Earth and Environmental Sciences, Department of Earth and Environmental Sciences
Funders: Natural Environment Research Council
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QD Chemistry


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