Development of Photobiomodulation (PBM) approaches for the management of oral diseases

Serrage, Hannah ORCID: 0000-0002-4995-6076 (2019). Development of Photobiomodulation (PBM) approaches for the management of oral diseases. University of Birmingham. Ph.D.

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Abstract

Photobiomodulation (PBM) is a non-invasive phototherapy that has shown promise in the treatment of the chronic oral inflammatory disease, periodontitis. This project aimed to provide evidence of a well characterised system for the evaluation of the effects of PBM on the most abundant cell type in the oral periodontium: primary human gingival fibroblasts (pHGFs).

Two bespoke high throughput light arrays were extensively characterised (2nd generation array: 400-830 nm, 24mW/cm² and LUMOS® array: 405-850 nm, 24mW/cm²) using techniques including beam profilometry and UV-Vis spectrometry. The biological effects of PBM on pHGFs (either single isolates/pooled from 3 individuals) were evaluated following application of media or periodontal disease relevant stimuli.

The effects of PBM were biomodulatory, where application of blue light (400-450 nm, 24mW/cm², 5.76J/cm²) to ‘healthy’ pHGFs induced increases in markers for inflammation (IL-8, ROS) but application of PBM to ‘disease’ pHGFs (bacterial stimulus applied) caused decreases in markers for inflammation. PBM also modulated mitochondrial activity, where pHGFs with a higher mitochondrial content exhibited greater maximal and basal respiratory rates.

These data indicate blue light could be an effective modality in the modulation of inflammation in periodontal disease. Results also provide novel evidence that mitochondrial content could be a prerequisite in ensuring a response to PBM in vivo.

Type of Work:	Thesis (Doctorates > Ph.D.)
Award Type:	Doctorates > Ph.D.
Supervisor(s):	Supervisor(s) Email ORCID Milward, Mike UNSPECIFIED orcid.org/0000-0002-9089-687X Cooper, Paul UNSPECIFIED orcid.org/0000-0003-1305-7287 Palin, William UNSPECIFIED orcid.org/0000-0003-2225-7635 Horstman, Pieter UNSPECIFIED UNSPECIFIED
Licence:	All rights reserved
College/Faculty:	Colleges (2008 onwards) > College of Medical & Dental Sciences
School or Department:	School of Dentistry
Funders:	Engineering and Physical Sciences Research Council, Other
Subjects:	Q Science > Q Science (General) R Medicine > RK Dentistry
URI:	http://etheses.bham.ac.uk/id/eprint/9700

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