The role of bacterial pore forming proteins in modulation of host cell function

Culverhouse, Nicole (2016). The role of bacterial pore forming proteins in modulation of host cell function. University of Birmingham. M.Sc.

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Abstract

Streptococcus pneumoniae is a commensal bacterium found in the nasopharynx. Under the right circumstances it can cause invasive disease so understanding the interactions between S. pneumoniae and the various types of host cells that it comes in contact with would aid in developing treatment options such new vaccines and new antibiotics to combat these diseases.

The aim of this project was to investigate how pneumolysin, the pore forming toxin produced by S. pneumoniae interacts with the host and the effect it has on host cells. A key aim of this project was to identify the pathways that are triggered in response to damage caused by pores formed from pneumolysin toxin and how these pathways help the cell resist cell death and aid in cell repair. Also of interest were the mechanisms by which pneumolysin aids in adherence to host cells, as invasion through epithelial and endothelial layers is the first step in invasion and subsequently disease.

This project resulted in the purification of various forms of pneumolysin and their lytic effects were tested on lung epithelial cells. The effects of pneumolysin on the cell tight junction proteins E-cadherin and actin was also assessed.

Type of Work:

Thesis (Masters by Research > M.Sc.)

Award Type:

Masters by Research > M.Sc.

Supervisor(s):