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The optimisation of the enzyme linked immunosorbant assay (ELISA) for thyroid peroxidase autoantibodies (TPO) autoantibodies (aAbs)

Cook, Stuart (2010)
M.Res. thesis, University of Birmingham.

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Abstract

The Enzyme Linked Immunosorbant Assay (ELISA) for Thyroid Peroxidase (TPO)autoantibodies (aAbs) is a useful clinical test for diagnosing human thyroid conditions. In this study experiments were carried out in order to improve two aspects of the TPO ELISA produced by The Binding Site Ltd. Firstly, modifications were made to the TPO ELISA in order to improve its calibration curve gradient. Secondly, the ability of the TPO ELISA to return consistent results over time was analysed and improved. All modifications made to the TPO ELISA during the study were to adhere to practical and economic restrictions made by The Binding Site Ltd. Results show that improvements to the calibration curve of the TPO ELISA could be made by modifying the way the assay is produced. However, these changes drastically increase the cost of the test, making them industrially unviable. It was also shown the reproducibility of the TPO ELISA was improved through the use of a national reference material.

Type of Work:M.Res. thesis.
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Chemistry
Subjects:QD Chemistry
Institution:University of Birmingham
ID Code:931
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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