Watts, Tim (2010)
Ph.D. thesis, University of Birmingham.
The work in this thesis used an in vitro flow system, which incorporated a capillary viscometer, to systematically determine the effects that haemodynamic variables, or rheological properties of the perfused, fluorescently-labelled blood, had on the behaviour of leukocytes and platelets. By varying shear rate and stress, blood haematocrit, and aggregation and deformability of red cells, we obtained evidence that leukocyte adhesion was dictated by the number of free-flowing cells 'marginated' near the vessel wall and the force experienced by their initial adhesive bonds. Platelet adhesion was dependent on the velocity of the free-flowing cells near the vessel wall and the force experienced by the platelets once adhered. In contrast to leukocytes, variation in platelet adhesion appeared less dependent on variation in the number of free-flowing cells near the vessel wall, but dictated more by the width of the peripheral plasma layer; a thinner plasma layer promoting more efficient platelet adhesion. Overall, this thesis provides evidence that the differential margination and adhesion of leukocytes and platelets is largely a result of the difference in their size. Thus, it seems that the sizes of RBC, leukocytes and platelets are all adapted to provide efficient immune and haemostatic responses in different regions of the circulation.
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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