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Switchable biological surfaces

Lashkor, Minhaj (2010)
M.Res. thesis, University of Birmingham.

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Abstract

The ability to control properties such as wettability and bio-molecule immobilisation onto self-assembled monolayers (SAMs) has many potential biological and medical applications. The aim of this project was to produce mixed biotinylated peptide monolayers that are responsive to an electric potential, thus providing a system in which the conformation of the biotinylated peptides could be switched. This allows for controlled protein immobilisation onto a mixed monolayer. Fluorescence images indicated that less binding took place between the neutravidin and the biotinylated peptide under a negative potential due to decreased image intensity. Control experiments were also carried out using non-biotinylated peptides to show there was minimal non-specific binding and that binding was only taking place on the biotin binding sites. Stability studies were also carried out using cyclic voltammetry on pure and mixed monolayers to further understand the stability range of the monolayers. High currents were observed in cyclic voltammograms of pure and mixed SAMs. In order to identify the cause of the high current readings, further samples were investigated of well known SAMs. Cyclic voltammograms of nitrophenolthiol and octadecanethiol suggested that a combination of polycrystalline gold and the use of PBS as an electrolyte caused excessive hydrogen evolution which overlapped with the reductive desorption peaks thus, generating high currents and unrealistic charge densities.

Type of Work:M.Res. thesis.
Supervisor(s):Preece, Jon Andrew and Mendes, Paula
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Chemistry
Subjects:QD Chemistry
Institution:University of Birmingham
ID Code:808
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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