eTheses Repository

Larval zebrafish electrocardiography electrodynmaic modelling and sensor design

Crowcombe, James Edward (2017)
Ph.D. thesis, University of Birmingham.

Loading
PDF (12Mb)Accepted Version

Abstract

This thesis presents the first model of the electrical activity of the larval zebrafish heart as well as the design and fabrication of novel electrode arrays that were created to measure the electrocardiogram. The model consists of realistic 3D geometry of a 3 day’s post fertilisation zebrafish heart and body with a bidomain electrical model that uses the Fitzhugh-Nagumo equations as the ionic model. The model is able to replicate experimentally observed conduction velocities and action potentials by using region specific parameters and simulate electrocardiograms that are comparable to measurements. The electrode arrays are constructed from flexible polyimide films with gold microelectrodes. These devices have the potential to improve the measurement of the electrocardiogram for drug screening applications as an alternative to the use of micropipette electrodes. Gold plating and PEDOT:PSS coating techniques were applied to the devices to successfully reduce electrode impedance with the effectiveness of each technique categorised using impedance spectroscopy. The devices were tested \(in\) \(vivo\) with larval zebrafish with limited success and so \(in\) \(vitro\) tests were conducted using an artificial current source.

Type of Work:Ph.D. thesis.
Supervisor(s):Tarte, Edward
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Electronic, Electrical and Systems Engineering
Subjects:TK Electrical engineering. Electronics Nuclear engineering
Institution:University of Birmingham
ID Code:7545
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
Export Reference As : ASCII + BibTeX + Dublin Core + EndNote + HTML + METS + MODS + OpenURL Object + Reference Manager + Refer + RefWorks
Share this item :
QR Code for this page

Repository Staff Only: item control page