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Development of novel molecular probes and synchrotron imaging techniques to study the uptake of metallo-cylinder drugs

Freer, Ashleigh Elizabeth (2017)
Ph.D. thesis, University of Birmingham.

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Abstract

Work described in this thesis shows the design and synthesis of functionalised, triple stranded, dinuclear, supramolecular helicates. Click chemistry has been utilized to functionalise the parent ligand with dansyl and coumarin moiety’s. These ligands have been used to successfully form four novel metal complexes upon reaction with iron(II) chloride. The stability and fluorescence of these complexes has been monitored. Imidazole ligands has been functionalised by the addition of alkyne and azide side chains to allow them to undergo click reactions. This modification has not been shown to impede complex formation, as the ligand have been utilized to form novel triple stranded iron(II) and nickel(II) complexes.
Synchrotron imaging has been used to produce elemental maps of cells treated with the parent metallo cylinder. This has allowed the uptake of the complex to be monitored. Two novel bromine functionalised cylinders has been developed to allow co-localisation experiments to be undertaken using XRF imaging. Cryo-SXT experiments have been undertaken to examine the morphology of HeK cells after treatment with parent metallo cylinder.

Type of Work:Ph.D. thesis.
Supervisor(s):Hannon, Michael J.
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Chemistry
Subjects:QD Chemistry
Institution:University of Birmingham
ID Code:7515
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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