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Novel approaches for image analysis of in vitro epithelial cultures with application to silver nanoparticle toxicity

Flight, Rachel Catharine (2017)
Ph.D. thesis, University of Birmingham.

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A novel imaging approach was developed for the purpose of counting cells from phase contrast microscopy images of laboratory grown (in vitro} cultures of epithelial cells. Validation through comparison with standard laboratory cell counting techniques showed this approach provided consistent and comparable results, whilst overcoming limitations of these existing techniques, such as operator variability and sample destruction.

The imaging approach was subsequently applied to investigate the effects of silver nanoparticles (AgNP} on H400 oral keratinocytes. Concurrent investigations into antimicrobial effects of AgNP were performed on Escherichia coli, Staphylococcus aureus and Streptococcus mutans to provide models for Gram-positive and Gram-negative infection, and to compare with the literature and oral keratinocyte toxicity. It was found that AgNP elicit size-, dose- and time-dependent growth inhibition in both human cells and bacteria, although bacterial inhibition was not achieved without significant cytotoxicity at the same concentrations.

Type of Work:Ph.D. thesis.
Supervisor(s):Cooper, Paul and Landini, Gabriel and Shelton, Richard M. and Styles, Iain B
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Chemistry, PSIBS Doctoral Training Centre
Subjects:QD Chemistry
RK Dentistry
TP Chemical technology
Institution:University of Birmingham
ID Code:7481
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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