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Development of robust expanded bed adsorption processes for CGMP manufacture of biopharmaceutical products

Ewert, Stephanie (2017)
Ph.D. thesis, University of Birmingham.

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Abstract

Expanded Bed Adsorption (EBA) is a form of liquid fluidized bed adsorption chromatography employing dense chromatographic media of defined size distribution. EBA's main advantage stems from its ability to perform chromatographic separations with crude feedstocks, thereby combining three separate tasks - clarification, concentration and initial capture/purification - in one single unit operation. Still, understanding of support particle movement (solid phase dispersion) is limited, impairing the process' robustness. In this work, the technique of Positron Emission Particle Tracking (PEPT) was employed extensively to study solid phase motion and dispersion in expanded beds of commercial media. In addition to providing evidence of classification and non-uniform bed expansion in all fluidised beds under study, PEPT was used to determine kinetics of bed stabilisation and identify changes in tracer position and speed along the length of the bed in response to variations in flow rate, degree of column misalignment, and means of fluid distribution. Furthermore, adsorbent particle motion was investigated under 'real process' conditions, i.e. during the application of a porcine serum feedstock.

Type of Work:Ph.D. thesis.
Supervisor(s):Thomas, Owen
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:Chemical Engineering
Subjects:TP Chemical technology
Institution:University of Birmingham
ID Code:6939
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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