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Development of Nanoparticles for Imaging Applications

Claire, Sunil (2016)
Ph.D. thesis, University of Birmingham.

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Abstract

Nanoparticles have attracted considerable recent research interest for a variety of applications and can be used to visualize and characterise biological processes at the molecular and cellular level. They can be exploited as contrast agents for imaging and functionalised to incorporate luminescent moieties to a provide multi-modal imaging capability. This thesis presents a detailed series of studies on a luminescently labelled gold and silica nanoparticles, with the use of optical and electron microscopies to examine their applications.

Using surfactant and peptide pre-coatings gold nanoparticles were coated with an organic dye developed bind to the nanoparticle surface. The imaging potential of these novel nanoprobes is explored by examining uptake in vitro in cancer cells. Confocal laser scanning and transmission electron microscopies indicate significant cellular uptake. Nuclear uptake is also observed and possible methods of translocation to the nucleus are discussed. Cell cycle dysregulation is also assessed with no statistically significant perturbations observed.

The cellular uptake of 13 nm and 100 nm gold nanoparticles coated with a luminescent ruthenium polypyridyl complex was also investigated. Microscopy studies demonstrated the imaging potential of luminescent nanoprobes upon cellular uptake which was not accompanied significant cytotoxicity.

Finally an investigation in to the interaction of luminescent silica particles with dentine is presented. Confocal and scanning electron microscopies enable qualitative comparisons of the dentinal tubule occluding ability of particles coated with surfactants. The data indicate surfactant hydrophobicity is important factor in governing interactions with the dentine and illustrate the potential of luminescence imaging in dentistry.

Type of Work:Ph.D. thesis.
Supervisor(s):Pikramenou, Zoe and Hodges, Nik and Styles, Iain B
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Chemistry
Additional Information:

Publication resulting from research: Claire, Sunil, et al. "The deposition and imaging of silica sub-micron particles in dentine." Journal of dentistry 43.10 (2015): 1242-1248. http://dx.doi.org/10.1016/j.jdent.2015.08.002

Keywords:luminescent, gold silica, nanoparticles, cells, dentine imaging
Subjects:QD Chemistry
Institution:University of Birmingham
ID Code:6574
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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