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Smart adsorption materials and systems for recovery of high value protein products

Cao, Ping (2016)
Ph.D. thesis, University of Birmingham.

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In this context, we produced thermoresponsive ionic exchange adsorbents via 'grafting from' polymerisation, and consequently applied them to a novel travelling cooling zone reactor (TCZR). Sepharose CL-6B, Superose 6 prep grade, and Superose 12 prep grade were selected as the base matrices in order to find the most suitable
particle size and pore diameter for the modification with thermoresponsive polymer. Both cation exchange adsorbents (thermoCEX) and anionic exchange adsorbents (thermoAEX) modified with poly(Nisopropylacrylamide) or pNIPAAm were introduced in Chapter 2, 3, and 5. All resins' binding affinity and maximum adsorption capacity increased with elevated temperature, however, the protein 'adsorption-desorption' behaviour of thermoCEX is much superior to that of thermoAEX. We also introduced cation exchange adsorbents with poly( ethylene glycol) or PEG in Chapter 4, although the thennoresponsiveness of thermoCEX-PEG-S6PG has been found even better than those of thermoCEX, the dynamic protein binding performance is limited due to its relatively lower protein binding capacity at higher temperature.

Type of Work:Ph.D. thesis.
Supervisor(s):Thomas, Owen
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Chemical Engineering
Additional Information:

Publications resulting from research:
Cao, Ping, et al. "Integrated system for temperature-controlled fast protein liquid chromatography. II. Optimized adsorbents and ‘single column continuous operation’." Journal of Chromatography A 1403, (2015): 118-31.

Müller, Tobias KH, et al. "Integrated system for temperature-controlled fast protein liquid chromatography comprising improved copolymer modified beaded agarose adsorbents and a travelling cooling zone reactor arrangement." Journal of Chromatography A 1285 (2013): 97-109.

Subjects:TP Chemical technology
Institution:University of Birmingham
ID Code:6450
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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