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Development of fluorophore-tagged DNA probes for cellular imaging applications

Bamford, Rosemary Anne (2015)
Ph.D. thesis, University of Birmingham.

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Abstract

Single nucleotide polymorphisms (SNPs) are single base variations in DNA which give genetic variation. However, SNPs can also be linked to the development of certain diseases. Modified oligonucleotides used to probe biological changes and processes have become an important focus of scientific research. Fluorescent tagging of DNA can be used to sense SNPs in DNA targets through differences in emission intensity on the formation of a duplex.
An anthracene-tagged DNA probe developed by Tucker \(et\) \(al\). is able to discriminate between a fully complementary DNA target sequence and one with a single base difference. This thesis describes how SNP sensing with anthracene-tagged DNA has been extended to SNPs in RNA targets and sequences associated with Alzheimer's disease. Finally, a new dual fluorophore DNA probe was designed for SNP sensing via FRET.

Type of Work:Ph.D. thesis.
Supervisor(s):Tucker, Jim and Wilkie, John and Dehghani, Hamid
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Chemistry
Subjects:QD Chemistry
QH426 Genetics
Institution:University of Birmingham
ID Code:6406
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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