Anthracene tagged biomolecules for DNA binding

Downloads

Downloads per month over past year

Bullen, Gemma Anne (2015). Anthracene tagged biomolecules for DNA binding. University of Birmingham. Ph.D.

[img]
Preview
Bullen15PhD.pdf
Text - Accepted Version
Available under License All rights reserved.

Download (10MB) | Preview

Abstract

Within this thesis, the use of anthracene to perform various applications within biomolecules is assessed. Anthracene displays two interesting photo properties which make it an appealing molecule for incorporation; fluorescence and photodimerisation. The former is utilised to develop a single nucleotide polymorphism detection assay which is shown to allow for determination of the base present in a complementary strand of DNA. In addition to this, the photodimerisation properties of anthracene are used within a protein for the first time. This is utilised to develop a photoswitched binding protein, allowing for control of DNA binding of the protein. Further to this, the photodimerisation properties are utilised within oligonucleotides to achieve structural control of a G-quadruplex as well as photo-triggered release of single stranded DNA.

Type of Work: Thesis (Doctorates > Ph.D.)
Award Type: Doctorates > Ph.D.
Supervisor(s):
Supervisor(s)EmailORCID
Peacock, AnnaUNSPECIFIEDUNSPECIFIED
Tucker, JamesUNSPECIFIEDUNSPECIFIED
Licence: All rights reserved
College/Faculty: Colleges (2008 onwards) > College of Engineering & Physical Sciences
School or Department: School of Chemistry
Funders: None/not applicable
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH426 Genetics
URI: http://etheses.bham.ac.uk/id/eprint/6369

Actions

Request a Correction Request a Correction
View Item View Item

Downloads

Downloads per month over past year