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Modified nucleic acids: structural studies and applications in biosensing

Carr-Smith, James (2015)
Ph.D. thesis, University of Birmingham.

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Abstract

The modification of natural nucleic acids or synthesis of novel DNA mimics can facilitate new structure, function and properties. In particular, the use of modified nucleic acids for applications in biosensing has become a popular field of study given the desire for rapid and reliable theranostic devices. The aim of the projects detailed in this thesis was to study a range of DNA modifications, with a view to gaining an enhanced understanding of their effects on DNA structure, but also on their ability to act as sensing platforms for the extraction of important biological information stored within DNA targets. The four projects discussed include: organometallic mimics of DNA based on ferrocene (FcNA) and corresponding FcNA-DNA conjugates and their effect on structure; FcNA-DNA conjugates that bind mercury; redox-active macrocycles incorporated into DNA as SNP sensors and DNA labeled virus particles that probe the presence of pathogens via a bionanoparticle supported PCR reaction which can be monitored by Linear Dichroism spectroscopy.

Type of Work:Ph.D. thesis.
Supervisor(s):Tucker, James
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Chemistry
Subjects:QD Chemistry
QH426 Genetics
Institution:University of Birmingham
ID Code:6273
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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