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Development and characterisation of chitosan and polyhydroxybutyrate based polymeric scaffolds

Blevins, Mark (2015)
Ph.D. thesis, University of Birmingham.

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Abstract

Electrospinning is a versatile method of producing nanofibrous polymeric material with potential applications as tissue engineering scaffolds. The main aim of this project was to produce and characterise electrospun polymeric scaffolds based on chitosan and bacterial polyhydroxybutyrate. The effect of the parameters used in the electrospinning process were studied and optimised by electrospinning polyvinyl alcohol from 8 wt% and 10 wt% solutions under a variable applied voltage from 10-25 kV. Attempts were made to electrospun chitosan however it was found that the creation of a polymer blend was necessary to facilitate fibre formation. PVA-chitosan blends were successfully electrospun at blend ratios of up to 80:20. A chitosan-hydroxybenzotriazole-PVA aqueous solution was successfully prepared enabling the production of chitosan/PVA nanofibres without the need for the use of an organic solvent. Polyhydroxybutyrate produced by bacterial synthesis from R. Eutropha using three different carbon sources; olive oil, rapeseed oil and glucose were electrospun and characterized. The choice of carbon source did not have a significant effect on the morphology or crystallinity of the produced fibres. PHB fibre diameters were reduced by 30% through the addition of the salt Benzyl tributylammonium chloride to the electrospinning solution.

Type of Work:Ph.D. thesis.
Supervisor(s):Stamboulis, Artemis
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Metallurgy and Materials
Subjects:QH301 Biology
R Medicine (General)
T Technology (General)
Institution:University of Birmingham
ID Code:5627
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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