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# Metabolism and transport of complex metabolites of mycobacteria

Varela Ramirex, Cristian (2014)
Ph.D. thesis, University of Birmingham.

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## Abstract

$$Mycobacterium$$ $$tuberculosis$$, the causative agent of the infectious disease Tuberculosis, is one of the most successful human pathogen. The disease remains a global health priority due to the spread of HIV and multidrug resistant strains. Therefore, there is a need to extend the understanding of the physiology and pathogenicity of $$M$$. $$tuberculosis$$ in order to develop new therapies, antimicrobial drugs and vaccines. $$Mycobacterium$$ possesses a unique cell envelope responsible for the reduced susceptibility to antibiotics and pathogenicity due to the high lipid content. It is composed by the plasma membrane, an unusual lipid-rich cell wall and an outermost layer known as the capsule. Moreover, other complex metabolites play a role in $$M.$$ $$tuberculosis$$ virulence such as inorganic polyphosphate, a polymer involved in stringent response and long term survival. In this study, a transposon mutant library was generated in order to identify new genetic determinants related to cell envelope; furthermore, specific mutants strains were generated to investigate the role of MmpL factors in mycolic acids transport, to test the role of a group of ABC-transporters in capsule biosynthesis and also to assess the function of exopolyphosphatases in survival under stress and nutrient limitation condition.

Type of Work: Ph.D. thesis. Bhatt, Apoorva Colleges (2008 onwards) > College of Life & Environmental Sciences School of Biosciences QR Microbiology University of Birmingham 5412
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