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The role of platelets in capturing circulating cells from flow in vivo an intravital microscopy study using a laser-induced model of thrombosis

Ashraf, Asma (2013)
M.Res. thesis, University of Birmingham.

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Abstract

Literature has increasingly shown how platelets may have roles beyond haemostasis. Platelet related health problems are seen in cancer patients on many occasions. Therefore, there is a possible relationship between platelets and cancer metastasising through the blood stream. Additionally, stem cell homing is an important aspect of stem cell therapy. For tissue regeneration to initiate, a sufficient number of stem cells must adhere to the injured sites. Present literature has shown that the low level of stem cell homing is a problem. Platelets are found at the site of injured tissues, so understanding the interactions between stem cells and platelets will enable research to be carried out to enhance homing mechanisms. In this study microcirculation was examined under an intravital microscope. A laser injury was induced causing damage to the blood vessels within the cremaster, which caused platelets to become adherent to the damaged area and form a thrombus. The labelled stem or tumour cells would then be perfused through in a single dose 1x106 cells when using haematopoietic stem cells or lewis lung carcinoma cells, and 5x105 cells when using mesenchymal stem cells. Results obtained demonstrated low levels of adherence between platelets and both types of stem cells and no adherence was observed between platelets and the tumour cells. Although this investigation has not provided any significant evidence of platelets interacting with stem cell or tumour cells, much literature has demonstrated their interactions and resulting therapeutic effects.

Type of Work:M.Res. thesis.
Supervisor(s):Kalia, Neena (Dr)
School/Faculty:Colleges (2008 onwards) > College of Medical & Dental Sciences
Department:School of Clinical and Experimental Medicine
Subjects:R Medicine (General)
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Institution:University of Birmingham
ID Code:4675
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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