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Modulatable endosomalytic, intracellularly biodegradable vectors for gene delivery

Nasanit, Rujikan (2010)
Ph.D. thesis, University of Birmingham.

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Abstract

In order to achieve efficient gene delivery, we have designed p\(K_a\) modulatable oligopeptides (2COPs) by combining with lysine, histidine and cysteine residues which will bind DNA extracellularly, internalize via endocytosis, provide a tunable endosomal release mechanism, and provide a degradable backbone in order that the DNA can be released once in the cytoplasm. The reducible polycations (RPCs) were synthesized from 2COPs. The sizes, surface charges and the stability of RPC polyplexes under the simulated physiological conditions extra- and intracellularly suggested that these RPCs are promising vectors. In addition, the transfections revealed that the RPCs can facilitate endosomal buffering and intracellular reduction and are non-toxic to cells. The nuclear targeting signal (TAT) was incorporated into these vectors. The reducible copolycations (RcPCs) were synthesized via oxidative polymerisation between 2COPs and TAT. The RcPC polyplexes are ~100 nm, and are positively charged. Gel shift assay revealed that RcPCs have less potential than RPCs to be used as vectors as they are less stable extracellularly than the RPCs. In addition, chloroquine was required to enhance the transfection of RcPCs. Furthermore, there is no improvement in transfection of RcPC compared to RPCs. Therefore, this suggests that the incorporation of TAT does not improve the transfection.

Type of Work:Ph.D. thesis.
Supervisor(s):Preece, Jon Andrew
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Chemistry
Subjects:QD Chemistry
QH301 Biology
Institution:University of Birmingham
ID Code:455
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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