eTheses Repository

Development and characterisation of polyhydroxybutyrate from selected bacterial species

Bagheriasl, Soroosh (2013)
Ph.D. thesis, University of Birmingham.

Loading
PDF (5Mb)Redacted Version

Abstract

In this study Serratia sp. NCIMB 40259 and R. eutropha H16 were utilised for the accumulation of Polyhydroxybutyrate(PHB). Serratia sp. with high phosphatase activity (HPA)or low phosphatase activity (LPA) were fermented in order to accumulate intracellular PHB and biomineralise hydroxyapatite (HA) on the cell surface. The optimum conditions for the accumulation of PHB, in this study, were found to be after 216 h fermentation, producing a PHB yield of 14.6 %w/w and 3.3 %w/w for LPA and HPA respectively. The molecular and thermal properties of PHB were observed to be largely unaffected by the phosphatase activity of the cells and appeared to be dependent on the fermentation period. R. eutropha cells were fermented with the substrates olive oil and rapeseed oil and compared with glucose. The use of either olive oil or rapeseed oil as carbon sources resulted in the greatest accumulation of PHB. The thermal and molecular properties of the PHB samples were found to be almost identical. The use of oils in the production of PHB would make a viable replacement for the use of sugars as substrates. Electrospun fibres of PHB from glucose, olive oil and rapeseed oil were found to have diameters dependant on their solution concentrations. By adjusting the electrospinning parameters it may be possible to control the crystallinity and the diameters the PHB fibres.

Type of Work:Ph.D. thesis.
Supervisor(s):Stamboulis, Artemis
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Metallurgy and Materials
Subjects:TN Mining engineering. Metallurgy
Institution:University of Birmingham
ID Code:4140
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
Export Reference As : ASCII + BibTeX + Dublin Core + EndNote + HTML + METS + MODS + OpenURL Object + Reference Manager + Refer + RefWorks
Share this item :
QR Code for this page

Repository Staff Only: item control page