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Lipids and metabolites detected by magnetic resonance spectroscopy as biomarkers in nervous system tumour cell lines

Pan, Xiaoyan (2013)
Ph.D. thesis, University of Birmingham.

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Nuclear magnetic resonance spectroscopy (NMR) resonances from lipids and metabolites in tumours are associated with tumour grade and treatment response. The origin of NMR lipid signal is mainly considered to be cytoplasmic lipid droplets (LDs). The aim of this study is to investigate the lipid species of LDs in nervous system tumour cells and identify potential lipidic or metabolic markers in treatment response. NMR spectroscopic analysis revealed that the LDs contain phosphatidylcholine, cholesterol and cholesterol ester with saturated, mono-unsaturated and polyunsaturated fatty acid species. Both saturated and unsaturated lipids are accumulated into LDs in cancer cell death. It is shown that Uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) and Uridine diphosphate N-acetylglucosamine galactosamine (UDP-GalNAc), the main donors of glycosylation, in parallel with 1H NMR detected lipids, increased in apoptotic cancer cells.LDs in nervous system cancer cell lines contain specific lipid species. To the best of our knowledge, it is the first study mechanistically links UDP-GlcNAc and UDP-GalNAc to cancer cell death.

Type of Work:Ph.D. thesis.
Supervisor(s):Peet, Andrew and Wilson, Martin and McConville, Carmel and Arvanitis, Theodoros N.
School/Faculty:Colleges (2008 onwards) > College of Medical & Dental Sciences
Department:School of Cancer Sciences
Subjects:RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Institution:University of Birmingham
ID Code:4093
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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