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Effects of composition on the properties of phospholipid bilayers

Burley, Andrew (2013)
Ph.D. thesis, University of Birmingham.

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The composition of biological phospholipid membranes has a significant effect on many processes which is as yet poorly understood. Dimyristoyl-phosphatidyl-serine (DMPS) and dimystristoyl-phosphatidyl-ethanolamine (DMPE) are commonly found in cell membranes. The properties of supported bilayers composed of mixtures of these two phospholipids were investigated using electrochemical techniques, infrared spectroscopy and neutron reflectometry.
Polarisation-modulated infrared reflectance spectroscopy investigations suggest that the lipid tail groups adopt one conform at 20% DMPS and a different conformation at 30% DMPS concentration in DMPE. Differential capacity measurements show that bilayers composed of 20% DMPS or less behave similarly to pure DMPE while at 40% DMPS concentrations or higher the bilayer behaves similarly to pure DMPS. Electrochemical impedance spectroscopy indicates that bilayers containing DMPS are more effective barriers to the passage of anions than DMPE bilayers: the opposite is true for cations. It is hypothesized that the negative charge residing on the DMPS head group plays a major role in these changes. Neutron reflectometry data indicate that a 10% DMPS bilayer gains water and finally detaches from the substrate when it is subjected to an increasing negative potential. These observations help explain the shape of the capacity curve with potential.

Type of Work:Ph.D. thesis.
Supervisor(s):Horswell, Sarah
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Chemistry
Subjects:QH301 Biology
Institution:University of Birmingham
ID Code:4055
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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