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Tetra-stranded metallo-supramolecular cylinders. design, synthesis and DNA binding studies

Calle Alonso, Natalia (2013)
Ph.D. thesis, University of Birmingham.

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The work described in this thesis concerns the design, synthesis, DNA binding and biological activity of palladium(II) supramolecular cylinders that might be capable of recognizing a DNA four-way junction.

An introduction to DNA structure will be presented, as well as the different binding modes of natural and synthetic agents that can recognise and bind to DNA. Since this work is focused on the design of large metallo-structures, the general principles of supramolecular chemistry will be summarised with particular emphasis on metallo-supramolecular structures.

Palladium(II) supramolecular cylinders have already been reported and these show promising cytotoxicity against different cancer cell lines. However, these complexes present poor solubility in aqueous solutions. It is therefore the aim of this thesis to improve the water solubility of palladium(II) supramolecular cylinders without significantly changing their structure or quenching their cytotoxic activity.

The DNA binding properties of the newly synthesised palladium(II) complexes will be presented. Several spectroscopic techniques, such as circular and linear dichroism and ethidium bromide displacement assays, as well as electrophoresis experiments were carried out and these will be discussed.

Initial DNA four-way junction experiments and cytotoxicity studies will also be discussed.

Type of Work:Ph.D. thesis.
Supervisor(s):Hannon, Michael J.
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Chemistry
Subjects:QH426 Genetics
TP Chemical technology
Institution:University of Birmingham
ID Code:4053
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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