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The effects of the human oocyte vestments and follicular fluid on spermatozoa

Frettsome, Rebecca Louise (2012)
Ph.D. thesis, University of Birmingham.

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Abstract

Our knowledge of the released human ovulatory components, the cumulus-oocyte complex (COC) and follicular fluid, and their physiological effects on spermatozoa and roles in fertilisation remain poorly characterised. The aim of this study was to use a multi-pronged approach to begin to unravel these interactions and their relation to fertilisation success. Experiments designed to better replicate the physiological environment of the female tract showed environmental modulation of sperm motility. Mean sperm velocity values VSL, VAP and VCL increased by 12.4%, 15%, 16.5% respectively, when exposed to cumulus cells from pregnancy-positive donors, compared to pregnancy-negative donors.
Follicular fluid elicited a [Ca2+]i response in spermatozoa that was independent of treatment outcome. The response of spermatozoa exposed to follicular fluid at a 50% (v/v) dilution was a large spike on the front of the ‘classical’ progesterone transient response, which has not been previously reported.
Human sperm-zona binding (SZB) studies are hampered by the shortage of oocytes, and thus zona pellucida (ZP) available for research. As a possible source of ZP this study investigates the development of an in vitro model for oogenesis, utilising follicular fluid and cumulus cell co-culture with human embryonic stem cells. The feasibility of SPR technology, using both native and recombinant sources of ZP, to measure SZB and identify possible binding candidates is also assessed.
The data in this study addresses just some of the potential effects of the COC and follicular fluid on spermatozoa. Further developments within this area may lead to better diagnostics and treatments for patients undergoing ART, in addition to providing targets for novel contraceptives.

Type of Work:Ph.D. thesis.
Supervisor(s):Connor, Sarah
School/Faculty:Colleges (2008 onwards) > College of Medical & Dental Sciences
Department:School of Clinical and Experimental Medicine
Subjects:R Medicine (General)
Institution:University of Birmingham
ID Code:3512
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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