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Cellular responses to dental extracellular matrix molecules

Smith, James George William (2012)
Ph.D. thesis, University of Birmingham.

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Abstract

Dental pulp contains mesenchymal stem cells (MSCs) similar to those present within bone marrow. Several factors are postulated to contribute to the signalling involved in regulating these cells. This project aimed to investigate the role of pulp and dentine extracellular matrix (pECM/dECM) in the regulation of pulp cell behaviour during health and disease. pECM/dECM molecules were extracted using 10% EDTA, pH7.2 followed by 0.5M-NaCl, pH11.7 and 0.1M tartaric acid, pH2.0, respectively containing protease inhibitors. Proteomic analysis demonstrated the complexity of the ECM extractions. pECM-coated cultureware reduced pulp cell proliferation rates and increased stem cell marker expression compared with controls. Pulp cells exhibited multipotential capacity, with pECM-coated culture surfaces enhancing differentiation activity. pECM and dECM promoted pulp cell migration through an active rho dependent pathway and the chemotactic effects of these ECM molecules were enhanced following acidic/proteolytic degradation. Recruited cells exhibited increased stem cell marker expression. dECM and pECM possessed demonstrable bacteriostatic activity against three anaerobic bacteria associated with dental disease. Dental pulp cells were shown to be viable and capable of secreting mineral when encapsulated within a pECM/alginate scaffold and exposed to dECM molecules. Dental ECMs play important roles in regulating cellular and tissue responses during health and disease.

Type of Work:Ph.D. thesis.
Supervisor(s):Smith, A J and Cooper, P R and Shelton, Richard M.
School/Faculty:Colleges (2008 onwards) > College of Medical & Dental Sciences
Department:School of Dentistry, Department of Oral Biology
Subjects:QH301 Biology
RK Dentistry
Institution:University of Birmingham
ID Code:3432
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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