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The role of galectin-3 and galectin-9 in the chronic inflammation of rheumatoid arthritis

Bik, Magadelena Anna (2009)
Ph.D. thesis, University of Birmingham.

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Abstract

Fibroblasts are important regulators of inflammatory processes. The phenotype of fibroblasts differ according to anatomical site which may dtermine immune functions such as leukocyte accumulation and predilection for inflammatory disease in certain tissues. This thesis describes the expression profile and explores the function of a family of immunomodulatory proteins (galectins) in fibroblasts from rhematoid arthritis patients. Synovial fibroblasts were found to differ significantly from bone marrow and skin fibroblasts with higher expression of galectin-9 and galectin-12 in synovial fibroblasts. Galectin-9 and galectin-3 expression was also examined in situ in synovial tissue from rheumatoid arthritis (RA) and osteoarthritis (OA) patients. Expression of both galectins were higher in RA synovial tissue compared to OA but not in synovial fibroblasts cultured in vitro. Galectin-3 expression seemed to be controlled by epigenetic factors (methylation) but not cytokine stimulation. Galectin-9 production was up-regulated by interferon-y, interleukin-1b and ligands for Toll-like receptors 3 (TLR3) and 4 (TLR4). It was found that intracellular presence of galectin-9 in RA synovial firboblasts increased their resistance to apoptosis. Galectin-3 level are increased in teh joints of patients with rheumatoid arthritis. Studies on the effect and mechanism of galectin-3 action on fibroblasts revealed that exogenously added galectin-3 induced production of cytokines (IL-6) from synovial and skin fibroblasts but the production of moncyte attracting chemokines (CCL5, CCL2) was induced uniquely in fibroblasts derived from teh synovium. Different signalling pathways mediated the secretion of those mediators. IL-6 release depended on MAP kinases p38, ERK and JNK as well as NFkB transcription factor, whereas CCL5 production required PI3/Akt and NFkB

Type of Work:Ph.D. thesis.
Supervisor(s):Lord, Janet and Buckley, Christopher D
School/Faculty:Colleges (2008 onwards) > College of Medical & Dental Sciences
Department:School of Immunity and Infection
Subjects:QR Microbiology
Institution:University of Birmingham
ID Code:332
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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