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Investigating the role of the cytoskeleton and signalling in the self-incompatibility response of Papaver rhoeas

Poulter, Natalie Sarah (2009)
Ph.D. thesis, University of Birmingham.

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Many flowering plants are hermaphrodite, which poses the problem of self-fertilisation and the subsequent loss of genetic fitness in the offspring. To prevent this, plants have developed a genetically controlled mechanism called self-incompatibility (SI) which allows self (incompatible) pollen to be recognised and rejected before fertilisation can occur. The SI response of Papaver rhoeas (field poppy) has been extensively studied at the molecular and cellular level. Rejection of incompatible pollen occurs on the stigma surface when the pollen S-determinant PrpS, a transmembrane protein, interacts with the stigmatic S-determinant, secreted S-proteins. This triggers a calcium-mediated signalling cascade that targets the cytoskeleton and results in programmed cell death (PCD) of incompatible pollen. Work presented in this thesis investigated the localisation of PrpS and S-proteins. Other studies investigated the role of the cytoskeleton in SI. These demonstrated the involvement of the microtubule cytoskeleton for the first time. Microtubules were rapidly depolymerised and this was implicated in signalling to PCD. The actin cytoskeleton has previously been shown to exhibit biphasic alterations during SI involving depolymerisation, followed by formation of F-actin foci. Studies described here represent the first steps toward characterisation of the F-actin foci. Their potential involvement in PCD and signalling is discussed.

Type of Work:Ph.D. thesis.
Supervisor(s):Franklin-Tong, Veronica
School/Faculty:Colleges (2008 onwards) > College of Life & Environmental Sciences
Department:School of Biosciences
Additional Information:

Appendix II is in two separate html files

Keywords:Self-incompatibility, pollen, actin cytoskeleton, microtubule cytoskeleton, programmed cell death
Subjects:QK Botany
Institution:University of Birmingham
ID Code:322
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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