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Investigating the effect of plant amino acid transporters AtAAP1 and AtAAP2 on aphid-plant interactions

Kemp, Harley (2011)
Ph.D. thesis, University of Birmingham.

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Abstract

A model system of Myzus persicae, Brevicoryne brassicae and Arabidopsis thaliana was used to investigate the effect of loss of function mutations in the plant amino acid transporter genes, AtAAP1 (Accession number: At1g58360) and AtAAP2 (Accession number: At5g09220). Homozygous mutant lines for each transporter were screened for phenotypic changes. Silique numbers and total silique seed mass were reduced for both aap1 and aap2 plants in comparison to wildtype plants (p < 0.05). Individual seed weight was also significantly reduced in aap2 plants (p < 0.05). Aphid probing behaviour, measured using EPG, indicated both aphid species took significantly longer attempting to locate a sieve element and reach sustained E2 feeding when on aap1 and aap2 plants (p < 0.05). The rate of aphid feeding was also significantly slower for both aphid species feeding on aap1 and aap2 (p < 0.05). M. persicae and B. brassicae feeding on aap1 and aap2 exhibited no change in aphid performance when compared to aphids on control plants (p > 0.05). Following antibiotic elimination of aphid symbionts in both species, aposymbiotic aphids were found to grow significantly slower on aap1 and aap2 plants in comparison with aposymbiotic aphids feeding on control plants (p < 0.005).

Type of Work:Ph.D. thesis.
Supervisor(s):Pritchard, Jeremy and Bale, Jeffrey S
School/Faculty:Colleges (2008 onwards) > College of Life & Environmental Sciences
Department:School of Biosciences
Subjects:QR Microbiology
Institution:University of Birmingham
ID Code:3169
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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