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Study of gigaseal formation in patch clamping using nanotechnology

Malboubi, Majid (2012)
Ph.D. thesis, University of Birmingham.

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Abstract

This PhD project is set out to study the mechanism of gigaseal’ formation and to provide techniques which enable the frequent formation of high resistance seals. Such techniques are highly desirable at both research and industrial levels. The research can be divided into three main sections. In the first section the most important factors in seal formation were identified. Then a group of novel approaches have been developed to alter these factors and achieve better sealing conditions. In the second section the effect of roughness, hydrophilicity and tip size on gigaseal’ formation has been studied. Patch clamp experiments were carried out using conventional and treated pipettes. In the third section of this research, glass micropipettes were characterized using various measurement techniques. The proposed techniques in this thesis not only explain the gigaseal formation in more detail, but also provide techniques to increase both the seal resistance and probability of seal formation. FIB polished pipettes improved the seal resistance significantly and chemically treated pipettes resulted in the formation of seals for more than 80% of trials. Therefore the research aims have been successfully met.

Type of Work:Ph.D. thesis.
Supervisor(s):Jiang, Kyle
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Mechanical Engineering
Subjects:TJ Mechanical engineering and machinery
TS Manufactures
Institution:University of Birmingham
ID Code:3137
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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