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The role of extracellular form of visfatin (eNAMPT) in modulating stress responses in cultured myocytes as a model of skeletal muscle ageing

Oita, Radu Cristian (2011)
Ph.D. thesis, University of Birmingham.

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Abstract

The understanding of the ageing process and of ageing-associated diseases represents a significant challenge for the scientific community, governments and society at large. I identified in skeletal muscle of murine models by microarray an increase in PPAR-β/δ expression during acute phase of hindlimb suspension (accelerated ageing), with a possible compensatory role, and an increase in expression levels of NR4A family of nuclear receptors in the skeletal muscle of caloric restricted rats (decelerated ageing). Adipose tissue has an endocrine role being actively involved in cross-talk with peripheral organs such as skeletal muscle. Visfatin is a recently discovered adipokine with pleiotropic functions. Unlike in other types of cells, in differentiated C2C12 myoblasts exogenous added visfatin (eNampt) did not act as an insulin-mimetic factor as shown by western blot and fluorescent assays. Visfatin treatment of differentiated C2C12 myotubes generated nevertheless an increase in the levels of reactive oxygen species as shown by fluorescent microscopy that was dependent on de novo transcription and translation of a new set of genes as revealed by RT-PCR. This increase in oxidative stress was independent of activation of the stress-activated protein kinases (MAPKs) such as ERK and p38, but dependent on NFkB activation as proved by western blot.

Type of Work:Ph.D. thesis.
Supervisor(s):Bunce, Christopher M.
School/Faculty:Colleges (2008 onwards) > College of Life & Environmental Sciences
Department:School of Biosciences
Subjects:QH301 Biology
Institution:University of Birmingham
ID Code:3086
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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